Three-dimensional cultures of human endometrial cells on Matrigel mimic in vivo morphology.
Three-dimensional cultures of human endometrial cells on Matrigel mimic in vivo morphology.
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发表时间：
2012-03
影响因子：
6.1
通讯作者：
Haiyan Zhu;Jun-xia Wang;X. Tong;Wei-hai Xu;Ling-ying Jiang;Xiao-ying Jing;Ling-yun Yang;F. Zhou;Song-Ying Zhang
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作者：
Haiyan Zhu;Jun-xia Wang;X. Tong;Wei-hai Xu;Ling-ying Jiang;Xiao-ying Jing;Ling-yun Yang;F. Zhou;Song-Ying Zhang
背景技术旁分泌效应器对子宫内膜生理学和形态发生的调节已通过体内研究得到充分确立。对子宫内膜功能的更全面的了解被推迟，部分原因是缺乏适当的培养模型。在本研究中，我们旨在使用 3-D 体外培养系统模拟子宫内膜细胞的体内三维 (3-D) 生长模式。方法将分离的子宫内膜上皮细胞、基质细胞和RL95-2细胞接种到涂有细胞外基质基质胶的培养室中，并用光学显微镜观察。使用荧光染色和免疫组织化学来评估形态学。结果根据培养条件，上皮细胞和RL95-2细胞在Matrigel上形成多细胞结构；基质细胞保持单独可区分或生长在一起形成 3D 晶格状结构。结论Matrigel为培养子宫内膜细胞提供了良好的微环境。在基质胶包被的室中培养的细胞与体内观察到的细胞非常相似。
BACKGROUND The regulation of endometrial physiology and morphogenesis by the paracrine effectors has been well established using in vivo studies. A more complete understanding of the endometrial function has been delayed due, in part, to a lack of appropriate culture models. In this study, we aimed to simulate the in vivo three-dimensional (3-D) growth pattern of endometrial cells using a 3-D in vitro culture system. METHODS Isolated endometrial epithelial cells, stromal cells and RL95-2 cells were seeded into culture chambers coated with the extracellular matrix Matrigel and observed using light microscopy. Fluorescence staining and immunohistochemistry were used to assess the morphology. RESULTS Depending on the culture conditions, epithelial cells and RL95-2 cells formed multicellular structures on Matrigel; stromal cells remained individually distinguishable or grew together to form 3-D lattice-like structures. CONCLUSIONS Matrigel provided a good microenvironment for culturing endometrial cells. The cells cultured in the Matrigel-coated chambers closely resembled those seen in vivo.