Ropivacaine myotoxicity after single intramuscular injection in rats
Ropivacaine myotoxicity after single intramuscular injection in rats
复制标题
DOI：
10.1017/s0265021505002036
复制
发表时间：
2006-02-01
影响因子：
3.6
通讯作者：
Vasilakos, D
中科院分区：
文献类型：
--
作者：
Amaniti, E;Drampa, F;Vasilakos, D
背景与目的：本研究旨在评价罗比卡因单次注射对大鼠的潜在肌肉毒性作用及其损伤的时程。方法：雄性Wistar大鼠128只，随机分为4组。前三组分别于右侧胫前肌注射0.75%罗比卡因、0.5%罗比卡因和生理盐水。第四组为不注射针刺组。于注射后第2、4、7、30天每组各处死8只。样本在光学显微镜下盲法检查是否有肌肉毒性的证据，评分为0=无损伤到3=肌坏死，并进行统计分析。注射后7d取材，进行透射电子显微镜检查。结果：0.75%罗比卡因和0.5%罗比卡因在第2、4、7天对肌纤维造成广泛破坏，与生理盐水组和针刺组比较，差异有统计学意义。除生理盐水组和针刺组外，其余各组注射药物对肌肉的损伤差异均有统计学意义。注射后30天，所有样本外观均恢复正常。结论：单次肌肉注射罗比卡因可引起可逆性肌肉损伤，且呈剂量依赖关系。
Background and objective: We conducted this study in order to evaluate the potential myotoxic effects of ropivacaine after single injection in rats and the time-course of the possible damage. Methods: One hundred and twenty-eight male Wistar rats were allocated to four different groups. The first three groups received intramuscular injections with ropivacaine 0.75%, ropivacaine 0.5% and normal saline, respectively, into the right tibialis anterior muscle. The fourth group received needle puncture without injection. Eight rats from each group were sacrificed 2, 4, 7 and 30 days after injection. Samples were blindly examined under light microscope for evidence of myotoxicity, scored as 0 = no damage to 3 = myonecrosis and statistically analysed. Samples obtained 7 days after injection were also examined under transmission electron microscope. Results: Ropivacaine 0.75% and ropivacaine 0.5% caused extensive destruction to muscles fibres, compared to saline or needle on days 2, 4 and 7. Statistically significant differences were found in muscle damage by drug injections among all groups except for saline vs. needle groups. Thirty days after injections all sample appearances had returned to normal. Conclusions: Ropivacaine after single intramuscular injection caused reversible muscle damage in a dose-dependent manner.