Transcriptional profiling in response to inhibition of cellulose synthesis by thaxtomin A and isoxaben in Arabidopsis thaliana suspension cells
Transcriptional profiling in response to inhibition of cellulose synthesis by thaxtomin A and isoxaben in Arabidopsis thaliana suspension cells
复制标题
DOI：
10.1007/s00299-009-0670-x
复制
发表时间：
2009-05-01
影响因子：
6.2
通讯作者：
Beaudoin, Nathalie
中科院分区：
文献类型：
--
作者：
Duval, Isabelle;Beaudoin, Nathalie
植物细胞壁决定细胞形状，是抵御环境挑战的主要屏障。细胞壁中纤维素含量的扰动导致细胞内稳态的整体变化，如纤维素合酶突变体或抑制纤维素合成后所见。特别地，应用纤维素合成抑制剂如thaxtomin A（TA）和异恶酰苯（IXB）在拟南芥悬浮细胞中启动依赖于从头基因转录的程序性细胞死亡（PCD）。为了进一步了解TA和IXB如何激活PCD，对从暴露于TA和IXB的拟南芥悬浮细胞分离的mRNA进行全基因组微阵列分析。超过75%的基因上调TA也上调IXB，包括基因编码细胞壁相关和钙结合蛋白，防御/压力相关的转录因子，信号传导组件和细胞死亡相关蛋白。与已发表的转录分析的比较显示，这些基因中有一半也诱导臭氧，创伤，细菌诱导子，Yariv试剂，几丁质和H2 O2。这些数据表明，IXB和TA都激活了类似的基因表达谱，其中包括通常响应于各种生物和非生物胁迫而诱导的基因的重要子集。然而，典型的基因激活的防御反应介导的经典水杨酸，茉莉酸或乙烯信号转导途径没有上调响应TA和IXB。这些结果表明，抑制纤维素合成诱导PCD的激活常见的压力相关的途径，将以某种方式绕过经典的依赖于防御途径。
The plant cell wall determines cell shape and is the main barrier against environmental challenges. Perturbations in the cellulose content of the wall lead to global modifications in cellular homeostasis, as seen in cellulose synthase mutants or after inhibiting cellulose synthesis. In particular, application of inhibitors of cellulose synthesis such as thaxtomin A (TA) and isoxaben (IXB) initiates a programmed cell death (PCD) in Arabidopsis thaliana suspension cells that is dependent on de novo gene transcription. To further understand how TA and IXB activate PCD, a whole genome microarray analysis was performed on mRNA isolated from Arabidopsis suspension cells exposed to TA and IXB. More than 75% of the genes upregulated by TA were also upregulated by IXB, including genes encoding cell wall-related and calcium-binding proteins, defence/stress-related transcription factors, signalling components and cell death-related proteins. Comparisons with published transcriptional analyses revealed that half of these genes were also induced by ozone, wounding, bacterial elicitor, Yariv reagent, chitin and H2O2. These data indicate that both IXB and TA activate a similar gene expression profile, which includes an important subset of genes generally induced in response to various biotic and abiotic stress. However, genes typically activated during the defence response mediated by classical salicylic acid, jasmonate or ethylene signalling pathways were not upregulated in response to TA and IXB. These results suggest that inhibition of cellulose synthesis induces PCD by the activation of common stress-related pathways that would somehow bypass the classical hormone-dependent defence pathways.