Multiple actions of 5‐hydroxytryptamine on myenteric neurones of the guinea‐pig ileum.
Multiple actions of 5‐hydroxytryptamine on myenteric neurones of the guinea‐pig ileum.
复制标题
5-羟色胺对豚鼠回肠肌间神经元的多重作用。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
R. North
中科院分区：
文献类型：
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作者：
S. Johnson;Y. Katayama;R. North
1.从豚鼠回肠肌间神经丛分离的神经节内的神经元进行细胞内记录。2.通过将5-羟色胺（5-HT）（200 nM-10 μ M）添加到灌注液中，将其应用于160个神经元。在27%的神经元中观察到去极化，并且更可能在S细胞中观察到。在30%的神经元中观察到超极化，并且更可能在AH细胞中观察到。52%的神经元未受影响。2. 5-HT通过微电泳从电极施加到104个神经元，电极的尖端靠近索马膜。在35%的细胞中观察到去极化，超极化13%; 10%的神经元在膜电位中表现出双相反应，其余细胞不受影响。4.对5-HT的去极化反应与神经元输入电阻的增加有关，可能是由于膜钾电导失活所致。在长时间或重复应用5-HT期间，反应幅度下降。5.对5-HT的超极化反应与神经元输入电阻的下降有关，可能是由于膜钾电导的激活。这些反应在整个5-HT应用期间持续存在，未显示出明显的快速耐受性。无钙溶液可可逆性消除超极化反应。6.对5-HT的去极化反应可能是豚鼠回肠中5-HT引起的乙酰胆碱释放的基础。超极化反应可能有助于抑制蠕动反射，当5-HT应用于肠的浆膜面时观察到了这一点。
1. Intracellular recordings were made from neurones lying within ganglia isolated from the myenteric plexus of the guinea‐pig ileum. 2. 5‐hydroxytryptamine (5‐HT) (200 nM‐10 microM) was applied to 160 neurones by adding it to the perfusing solution. Depolarizations were observed in 27% of neurones, and were more likely to be observed in S‐cells. Hyperpolarizations were observed in 30% of neurones, and were more likely to be seen in AH cells. 52% of neurones were unaffected. 2. 5‐HT was applied to 104 neurones by micro‐electrophoresis from an electrode with its tip placed close to the soma membrane. Depolarizations were observed in 35% of cells, and hyperpolarizations 13%; 10% of neurones showed biphasic responses in membrane potential and the remaining cells were unaffected. 4. The depolarizing responses to 5‐HT were associated with an increase in neuronal input resistance and were probably due to inactivation of the membrane potassium conductance. The responses declined in amplitude during prolonged or repeated applications of 5‐HT. 5. The hyperpolarizing responses to 5‐HT were associated with a fall in neuronal input resistance and were probably due to activation of the membrane potassium conductance. These responses persisted throughout the duration of application of 5‐HT and did not show marked tachyphylaxis. The hyperpolarizing responses were reversibly abolished by calcium‐free solutions. 6. The depolarizing responses to 5‐HT may underlie the release of acetylcholine caused by 5‐HT in the guinea‐pig ileum. The hyperpolarizing responses may contribute to the inhibition of the peristaltic reflex which has been observed when 5‐HT is applied to the serosal aspect of the intestine.