Evaluation of metabolomics behavior of human colon cancer HT29 cell lines treated with ionic liquid graviola fruit pulp extract
Evaluation of metabolomics behavior of human colon cancer HT29 cell lines treated with ionic liquid graviola fruit pulp extract
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DOI：
10.1016/j.jep.2021.113813
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发表时间：
2021-01-17
影响因子：
5.4
通讯作者：
Elnour, Ahmed A. M.
中科院分区：
文献类型：
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作者：
Daddiouaissa, Djabir;Amid, Azura;Elnour, Ahmed A. M.
民族药理学相关性：几个世纪以来，世界各地的土著人民一直使用药用植物来帮助人们保持健康和治疗疾病。番荔枝（Graviola）属于番荔枝科（Annonaceae），由于其药用能力包括抗微生物、抗炎、抗氧化和癌细胞生长抑制而传统上被使用。由于其对几种癌细胞系的选择性细胞毒性，Graviola被认为是一种潜在的抗肿瘤药物。然而，其抗癌活性的代谢机制信息仍然有限。本研究的目的：本研究旨在通过使用非靶向的基于GC-TOFMS的代谢谱来研究离子液体-Graviola果浆提取物（IL-GPE）对结肠癌（HT 29）代谢组学行为的影响。材料和方法：多变量数据分析用于确定代谢谱，用独创性通路分析（IPA）预测IL GPE和Taxol处理HT 29细胞后改变的经典通路结果：主成分分析（PCA）鉴定出44种具有最可靠因子负荷的代谢物，聚类分析（CA）将代谢物分为3组：未处理的HT 29细胞特异性代谢物、用粗制IL-GPE处理的HT 29细胞特异性代谢物和紫杉醇处理的特异性代谢物。代谢组学的途径分析表明，IL GPE粗品对能量代谢和肿瘤细胞增殖有明显的抑制作用，包括氨基酸代谢、有氧糖酵解、尿素循环和酮体代谢等。结论：IL GPE粗品对能量代谢和肿瘤细胞增殖有明显的抑制作用，有望成为一种有前景的抗癌药物。
Ethnopharmacological relevance: Medicinal plants have been used by indigenous people across the world for centuries to help individuals preserve their wellbeing and cure diseases. Annona muricata L. (Graviola) which is belonging to the Annonaceae family has been traditionally used due to its medicinal abilities including antimicrobial, anti-inflammatory, antioxidant and cancer cell growth inhibition. Graviola is claimed to be a potential antitumor due to its selective cytotoxicity against several cancer cell lines. However, the metabolic mechanism information underlying the anticancer activity remains limited.Aim of the study: This study aimed to investigate the effect of ionic liquid-Graviola fruit pulp extract (IL-GPE) on the metabolomics behavior of colon cancer (HT29) by using an untargeted GC-TOFMS-based metabolic profiling.Materials and methods: Multivariate data analysis was used to determine the metabolic profiling, and the ingenuity pathway analysis (IPA) was used to predict the altered canonical pathways after treating the HT29 cells with crude IL-GPE and Taxol (positive control).Results: The principal components analysis (PCA) identified 44 metabolites with the most reliable factor loading, and the cluster analysis (CA) separated three groups of metabolites: metabolites specific to the non-treated HT29 cells, metabolites specific to the treated HT29 cells with the crude IL-GPE and metabolites specific to Taxol treatment. Pathway analysis of metabolomic profiles revealed an alteration of many metabolic pathways, including amino acid metabolism, aerobic glycolysis, urea cycle and ketone bodies metabolism that contribute to energy metabolism and cancer cell proliferation.Conclusion: The crude IL-GPE can be one of the promising anticancer agents due to its selective inhibition of energy metabolism and cancer cell proliferation.