DRMY1 promotes robust morphogenesis by sustaining translation of a hormone signaling protein.
DRMY1 promotes robust morphogenesis by sustaining translation of a hormone signaling protein.
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DRMY1 通过维持激素信号蛋白的翻译来促进稳健的形态发生。
DOI：
10.1101/2023.04.07.536060
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Roeder,AdrienneHK
中科院分区：
文献类型：
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作者：
Kong,Shuyao;Zhu,Mingyuan;Scarpin,MRegina;Pan,David;Jia,Longfei;Martinez,RyanE;Alamos,Simon;Vadde,BatthulaVijayaLakshmi;Garcia,HernanG;Qian,Shu-Bing;Brunkard,JacobO;Roeder,AdrienneHK
稳健性是指尽管环境变化和遗传扰动，表型的不变发展。在拟南芥花芽中，四个萼片坚固地萌生并生长到恒定的大小以包围和保护内部花器官。我们之前描述了与突变体发育相关的 myb-like 1 (drmy1)，其中 3-5 个萼片不同程度地起始并生长到不同的大小，从而损害了它们的保护功能。这种鲁棒性丧失的分子机制尚不清楚。在这里，我们表明 drmy1 降低了雷帕霉素靶标 (TOR) 活性、核糖体含量和翻译。翻译减少降低了 ARABIDOSISRESPONSE REGULATOR7 (ARR7) 和 ARABIDOPSISHISTIDINE PHOSPHOTRANSFER PROTEIN 6 (AHP6) 的蛋白质水平，这两种细胞分裂素信号传导抑制剂通常在萼片起始之前快速产生。由此产生的细胞分裂素信号传导的上调破坏了强大的生长素模式和萼片起始。我们的工作表明，翻译的稳态是一种普遍存在的细胞过程，对于器官发生的稳健时空模式至关重要。
Robustness is the invariant development of phenotype despite environmental changes and genetic perturbations. In theArabidopsisflower bud, four sepals robustly initiate and grow to a constant size to enclose and protect the inner floral organs. We previously characterized the mutantdevelopment-related myb-like 1(drmy1), where 3–5 sepals initiate variably and grow to different sizes, compromising their protective function. The molecular mechanism underlying this loss of robustness was unclear. Here, we show thatdrmy1has reduced TARGET OF RAPAMYCIN (TOR) activity, ribosomal content, and translation. Translation reduction decreases the protein level ofARABIDOPSISRESPONSE REGULATOR7 (ARR7) andARABIDOPSISHISTIDINE PHOSPHOTRANSFER PROTEIN 6 (AHP6), two cytokinin-signaling inhibitors that are normally rapidly produced before sepal initiation. The resultant upregulation of cytokinin signaling disrupts robust auxin patterning and sepal initiation. Our work shows that the homeostasis of translation, a ubiquitous cellular process, is crucial for the robust spatiotemporal patterning of organogenesis.