PHYLOGENETICALLY CONSERVED SEQUENCES IN THE PROMOTER OF THE RABBIT SODIUM-HYDROGEN EXCHANGER ISOFORM-1 GENE (NHE1/SLC9A1)
PHYLOGENETICALLY CONSERVED SEQUENCES IN THE PROMOTER OF THE RABBIT SODIUM-HYDROGEN EXCHANGER ISOFORM-1 GENE (NHE1/SLC9A1)
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DOI：
10.1016/0167-4781(95)00075-r
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发表时间：
1995-06-09
期刊：
影响因子：
--
通讯作者：
IGARASHI, P
中科院分区：
文献类型：
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作者：
BLAUROCK, MC;REBOUCAS, NA;IGARASHI, P
NHE1（基因符号 SLC9A1）编码阿米洛利敏感的 Na+-H+ 交换剂的亚型，存在于许多细胞和肾上皮的基底外侧膜中。 NHE1 的表达受到慢性代谢性酸中毒、生长因子和佛波醇摄入者的调节。为了开始检查这种调节的分子基础，获得了包含 6 kb 5' 侧翼区域、第一个外显子和 NHE1 第一个插入序列的一部分的兔基因组克隆。天然兔组织中的主要转录起始位点位于起始密码子 5' 798 bp 处。兔 NHE1 近端 5' 侧翼区域的序列与人类序列相似，包含 TATA 盒、(G + C) 盒、同嘧啶同向重复序列和推定的 AP-1 位点。当连接到荧光素酶并转染到猪肾上皮细胞 (LLC-PK1) 中时，近端 5' 侧翼区域的 708 bp 表现出方向依赖性启动子活性。
NHE1 (gene symbol SLC9A1) encodes an isoform of the amiloride-sensitive Na+-H+ exchanger that is present in many cells and the basolateral membrane of renal epithelia. Expression of NHE1 is modulated in response to chronic metabolic acidosis, growth factors, and phorbol eaters. To begin examining the molecular basis for this regulation, a rabbit genomic clone that contained 6 kb of 5' flanking region, the first exon, and a portion of the first intervening sequence of NHE1 was obtained. The principal transcription start site in native rabbit tissues was located 798 bp 5' to the initiation codon. The sequence of the proximal 5' flanking region of rabbit NHE1 was similar to the human sequence and contained a TATA-box, (G + C)-boxes, homopyrimidine direct repeats, and a putative AP-1 site. When ligated to luciferase and transfected into porcine renal epithelial cells (LLC-PK1), 708 bp of proximal 5' flanking region exhibited orientation-dependent promoter activity.