IMMUNOHISTOCHEMICAL LOCALIZATION OF SUBSTANCE-P RECEPTOR IN THE CENTRAL-NERVOUS-SYSTEM OF THE ADULT-RAT
IMMUNOHISTOCHEMICAL LOCALIZATION OF SUBSTANCE-P RECEPTOR IN THE CENTRAL-NERVOUS-SYSTEM OF THE ADULT-RAT
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DOI：
10.1002/cne.903470208
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发表时间：
1994-09-08
影响因子：
2.5
通讯作者：
MIZUNO, N
中科院分区：
文献类型：
--
作者：
NAKAYA, Y;KANEKO, T;MIZUNO, N
为了揭示P物质(SP)在中枢神经系统(CNS)中的作用部位，用免疫细胞化学方法研究了SP受体在成年大鼠的分布，并与SP阳性纤维进行了比较。SPR样免疫反应主要定位于神经元胞体和树突。SPR-LI阳性神经元密集分布于杏仁皮质核、齿状回门部、蓝斑、疑核吻侧半部和胸髓中间外侧核，中等强度分布于尾壳核、伏隔核、嗅结节、正中核、桥脑和中缝大核，三叉神经脊束核尾侧亚核的I、III层和脊髓I层，大脑皮质、中脑基底核、屏状核、巨细胞网状核和小脑IX、X小叶。弱至中度SPR-LI的神经元在中枢神经系统中的分布更为广泛。SPR-LI的区域分布模式与SP阳性纤维不一定相同。脚内核、黑质和脚间核外侧部分有较强的SP-LI，但几乎没有SPR-LI。相反，齿状回门部、丘脑前背侧核、下丘中央核和被盖背侧核呈强至中等强度的SPR-LI，但含有少量SP-LI的轴突。这些发现证实了SP和SPR本地化之间存在“不匹配”的问题。然而，SPR-LI的分布与SP结合活性的分布非常一致，这已通过放射自显影进行了研究。这表明，本研究中揭示的SPR-LI位点代表了SP结合活性的大部分(如果不是全部)。(C)1994年Wiley-Liss，Inc.
In an attempt to reveal the function sites of substance P (SP) in the central nervous system (CNS), the distribution of SP receptor (SPR) was immunocytochemically investigated in adult rat and compared with that of SP-positive fibers. SPR-like immunoreactivity (LI) was mostly localized to neuronal cell bodies and dendrites. Neurons with intense SPR-LI were distributed densely in the cortical amygdaloid nucleus, hilus of the dentate gyrus, locus ceruleus, rostral half of the ambiguus nucleus, and intermediolateral nucleus of the thoracic cord; moderately in the caudatoputamen, nucleus accumbens, olfactory tubercle, median, pontine, and magnus raphe nuclei, laminae I and III of the caudal subnucleus of the spinal trigeminal nucleus, and lamina I of the spinal cord; and sparsely in the cerebral cortex, basal nucleus of Meynert, claustrum, gigantocellular reticular nucleus, and lobules IX and X of the cerebellar vermis. Neurons with weak to moderate SPR-LI were distributed more widely throughout the CNS. The regional patterns of distribution of SPR-LI were not necessarily the same as those of SP-positive fibers. The entopeduncular nucleus, substantia nigra, and lateral part of the interpeduncular nucleus showed intense SP-LI but displayed almost no SPR-LI. Conversely, the hilus of the dentate gyrus, anterodorsal thalamic nucleus, central nucleus of the inferior colliculus, and dorsal tegmental nucleus showed intense to moderate SPR-LI but contained few axons with SP-LI. These findings confirmed the presence of the ''mismatch'' problem between SP and SPR localizations. However, the distribution of SPR-LI was quite consistent with that of the SP-binding activity, which has been studied via autoradiography. This indicates that the sites of SPR-LI revealed in the present study represent most, if not all, sites of SP-binding activity. (C) 1994 Wiley-Liss, Inc.