A vector system for efficient and economical switching of a ura4+ module to three commonly used antibiotic marker cassettes in Schizosaccharomyces pombe
A vector system for efficient and economical switching of a ura4+ module to three commonly used antibiotic marker cassettes in Schizosaccharomyces pombe
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DOI：
10.1002/yea.3088
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发表时间：
2015-11
期刊：
影响因子：
2.6
通讯作者：
Ying-Hui Chen;Lihua Chen;Ke An;Yamei Wang;Q. Jin
中科院分区：
文献类型：
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作者：
Ying-Hui Chen;Lihua Chen;Ke An;Yamei Wang;Q. Jin
我们在这里描述了一组质粒载体的发展，允许简单，有效和经济的开关的ura4+模块在现有的裂殖酵母菌菌株的任何三个常规使用的抗生素标记盒，kanMX6，hphMX6和natMX6。原则上，该系统的应用还可以扩展到将ura4+转换为其他基于MX6模块的盒，如bleMX6，只要抗生素标记已克隆到ura4+模块转换载体中。我们举例说明了这套载体在现有菌株中与三种抗生素标记盒交换ura4+标记中的应用。版权所有© 2015约翰威利父子有限公司.
We describe here the development of a set of plasmid vectors that allow simple, efficient and economical switching of a ura4+ module in existing Schizosaccharomyces pombe strains to any of the three routinely used antibiotic marker cassettes, kanMX6, hphMX6 and natMX6. In principle, the applications of this system can also be extended to switching ura4+ for additional MX6 module‐based cassettes, such as bleMX6, as long as the antibiotic marker has been cloned into an ura4+ module‐switching vector. We illustrate the application of this set of vectors in exchange of the ura4+ marker in existing strains with three antibiotic marker cassettes with high efficiency. Copyright © 2015 John Wiley & Sons, Ltd.