Impact of Glyphosate on the Rhizosphere Microbial Communities of An EPSPS-Transgenic Soybean Line ZUTS31 by Metagenome Sequencing.
Impact of Glyphosate on the Rhizosphere Microbial Communities of An EPSPS-Transgenic Soybean Line ZUTS31 by Metagenome Sequencing.
复制标题
通过宏基因组测序研究草甘膦对 EPSPS 转基因大豆品系 ZUTS31 根际微生物群落的影响
DOI：
10.2174/1389202918666170705162405
复制
发表时间：
2018-01
期刊：
影响因子：
2.6
通讯作者：
Yang YH
中科院分区：
文献类型：
--
作者：
Lu GH;Hua XM;Cheng J;Zhu YL;Wang GH;Pang YJ;Yang RW;Zhang L;Shou H;Wang XM;Qi J;Yang YH
背景：草甘膦在世界范围内的使用急剧增加，但也引起了人们对其对矿物质营养、植物病原体和土壤微生物群的影响的关注。迄今为止，之前的大量研究仍然对草甘膦施用对土壤根际微生物群落的影响得出了不同的结果。目的：研究草甘膦对大豆epsps转基因品系ZUTS31一个生长季节的固氮、抑菌抑病及根际微生物群落的影响。方法：采用16S rRNA基因扩增子测序和霰弹枪宏基因组测序方法，对灌种期喷施稀释草甘膦溶液的大豆品系ZUTS31叶片与仅喷施水的大豆品系进行土壤根际微生物群落对比分析。结果：经草甘膦处理后，大豆根际细菌α多样性差异不显著，β多样性差异不显著。显著丰富的基因本体（GO）术语是生物过程的细胞、代谢和单生物，以及分子功能的结合、催化活性。草甘膦处理后，根际中与植物生长促进性状（PGPT）相关的部分功能基因，尤其是大部分固氮基因的命中数和基因丰度显著降低。结论：本研究表明，草甘膦-异丙胺盐配方对大豆品系ZUTS31根际细菌群落的α和β多样性没有显著影响，但在单生长季节对根际一些与PGPT相关的功能基因有显著影响。
Background: The worldwide use of glyphosate has dramatically increased, but also has been raising concern over its impact on mineral nutrition, plant pathogen, and soil microbiota. To date, the bulk of previous studies still have shown different results on the effect of glyphosate application on soil rhizosphere microbial communities. Objective: This study aimed to clarify whether glyphosate has impact on nitrogen-fixation, pathogen or disease suppression, and rhizosphere microbial community of a soybean EPSPS-transgenic line ZUTS31 in one growth season. Method: Comparative analysis of the soil rhizosphere microbial communities was performed by 16S rRNA gene amplicons sequencing and shotgun metagenome sequencing analysis between the soybean line ZUTS31 foliar sprayed with diluted glyphosate solution and those sprayed with water only in seed-filling stage. Results: There were no significant differences of alpha diversity but with small and insignificant difference of beta diversity of soybean rhizosphere bacteria after glyphosate treatment. The significantly enriched Gene Ontology (GO) terms were cellular, metabolic, and single-organism of biological process together with binding, catalytic activity of molecular function. The hits and gene abundances of some functional genes being involved in Plant Growth-Promoting Traits (PGPT), especially most of nitrogen fixation genes, significantly decreased in the rhizosphere after glyphosate treatment. Conclusion: Our present study indicated that the formulation of glyphosate-isopropylamine salt did not significantly affect the alpha and beta diversity of the rhizobacterial community of the soybean line ZUTS31, whereas it significantly influenced some functional genes involved in PGPT in the rhizosphere during the single growth season.