Body mass index of pancreatic donors: A decisive factor for human islet isolation
Body mass index of pancreatic donors: A decisive factor for human islet isolation
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胰腺捐献者的体重指数： 人体胰岛分离的决定性因素
DOI：
10.1055/s-0029-1211388
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发表时间：
1995-01-01
影响因子：
1.8
通讯作者：
Bretzel, RG
中科院分区：
文献类型：
--
作者：
Brandhorst, H;Brandhorst, D;Bretzel, RG
尽管在胰岛分离技术的改进，胰岛移植作为一种可靠地使人I型糖尿病受体血糖正常的方法仍然是不可预测的。免疫抑制预防原发性无功能、促进植入和预防排斥反应的进展应能提高成功率。然而，影响隔离过程的因素仍然没有完全确定。在我们的经验中，供者的营养状况以及其他供者特征被认为与胰岛分离成功相关。因此，在这项研究中，我们试图澄清人体胰腺供体的体重指数是否影响胰岛分离产量和活力。在瘦供体中，我们发现与正常和肥胖供体相比胰岛产量显著降低，与肥胖供体相比胰岛活力显著降低。肥胖供体胰岛的胰岛素分泌能力显著高于瘦供体和正常供体胰岛。总之，选择来自与BMI > 24相关的肥胖供体的胰腺用于胰岛制备提高了胰岛产量和活力。我们推测，一方面，肥胖供体胰腺中脂肪分布的增加可能有助于胶原酶消化过程中胰岛的释放，另一方面，肥胖供体的胰腺比瘦供体的胰腺含有更多的胰岛。这些数据强调了胰腺供体的体重指数对成功的人胰岛分离的决定性影响。体重指数应被视为胰岛制备成功的潜在预测因素。
Despite improvements in islet isolation techniques, islet transplantation remains unpredictable as a method for reliably rendering human type I diabetic recipients normoglycemic. Advances in immunosuppression to prevent primary nonfunction, to promote engraftment and to prevent rejection should improve success rates. However, factors influencing the isolation process remain incompletely defined. During our experience, the donor's nutritional status as well as other donor characteristics were noted to be associated with islet isolation success. Thus, in this study, we tried to clarify whether the body mass index of the human pancreatic donor affects islet isolation yield and viability. In lean donors we found significantly lower islet yields in comparison with normal and obese donors and a significantly lower islet viability compared to obese donors. Obese donor islets had a significantly higher insulin secretory capacity than lean and normal donor islets. In summary, islet yield and viability were improved selecting pancreata from obese donors associated with a BMI > 24 for islet preparation. We hypothesize that, on the one hand, the increased distribution of fat in pancreata of obese donors possibly can facilitate the release of islets during the collagenase digestion, and, on the other hand, pancreata of obese donors contain more islets than pancreata of lean donors. These data underline the decisive influence of the pancreas donor's body mass index on successful human islet isolation. The body mass index should be noted as a potential predictor of success of islet preparations.