Identification and characterization of Pseudomonas aeruginosa PA-IIL lectin gene and protein compared to PA-IL
Identification and characterization of Pseudomonas aeruginosa PA-IIL lectin gene and protein compared to PA-IL
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DOI：
10.1111/j.1574-695x.2000.tb01505.x
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发表时间：
2000-09-01
影响因子：
--
通讯作者：
Garber, NC
中科院分区：
文献类型：
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作者：
Gilboa-Garber, N;Katcoff, DJ;Garber, NC
利用铜绿假单胞菌ATCC 33347的岩藻糖/甘露糖结合的33个N端氨基酸：凝集素PA-IIL，在GenBank中检索铜绿假单胞菌PAO 1基因组序列，发现一个编码114个氨基酸的蛋白质(不包括启动子蛋氨酸)与该氨基酸序列完全匹配。在其终止密码子之后，有一个富含GC的序列，具有完美的染色对称性，促进发夹环结构的形成，可能使Rho不依赖转录终止。在推测的核糖体结合位点的上游有类似于费氏弧菌Luxi box的序列，与该基因的自我诱导一致。预测的PA-IIL分子量经质谱学证实为11732Da。其等电点为3.88。C-末端结构域特别疏水，意味着可能嵌入细胞膜。PA-IL与铜绿假单胞菌PA-IL在某些氨基酸和潜在糖基化位点上相似，但缺乏半胱氨酸、蛋氨酸和组氨酸。尽管它们在功能和调控上存在联系，但它们的基因却存在很大的差异(约867.5 kb)。(C)2000年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
Using the 33 N-terminal amino acids of the fucose/mannose binding: lectin PA-IIL of Pseudomonas aeruginosa ATCC 33347 in a tblastn search of P. aeruginosa PAO1 genomic sequence in GenBank revealed a single open reading frame encoding a 114-amino acid protein (excluding initiator methionine) perfectly matching that amino acid sequence. following its stop codon there is a GC-rich sequence having a perfect dyed symmetry promoting formation of a hairpin loop structure, potentially enabling rho-independent transcription termination. Upstream of the putative ribosomal binding site there are sequenxces resembling Vibrio fischeri luxI box, consistent with autoinduction of this gene. The predicted PA-IIL molecular mass, confirmed by mass spectrometry, is 11 732 Da. Its pI is 3.88. The C-terminal domain is particularly hydrophobic, implying possible embedding in the cell membrane. PA-IIL is similar to P, aeruginosa PA-IL lectin in some amino acids and potential glycosylation sites but lacks cysteine, methionine and histidine. Despite their relations in functions and regulation, their genes are widely separated (by about 867.5 kb). (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.