Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry.
Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry.
复制标题
通过流式细胞术表征细胞毒性 T 淋巴细胞的表型和功能。
DOI：
10.1007/978-1-4939-1158-5_3
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
McDyer,John
中科院分区：
文献类型：
--
作者：
Popescu,Iulia;Pipeling,Matthew;Akulian,Jason;McDyer,John
细胞毒性T淋巴细胞（CTL）是适应性免疫系统的重要组成部分。除了肿瘤监测之外，CTL的发展对于细菌和病毒感染特别重要。测量T细胞免疫功能在评估宿主防御、变态反应、自身免疫、移植排斥和肿瘤免疫中是重要的。近年来，已经可以测量单个细胞中的多种效应子功能，如细胞因子、转录因子和细胞溶解功能。此外，这些参数可以结合细胞增殖进行评估。在本章中，我们详细介绍了这些基于细胞的检测和方法，用于表征和量化CTL的表型和功能。
Cytotoxic T lymphocytes (CTLs) are important constituents of the adaptive immune system. Development of CTLs are particularly important for bacterial and viral infections, in addition to tumor surveillance. Measuring T cell immune function is important in evaluating host defense, allergy, autoimmunity, transplant rejection, and tumor immunity. In these recent years it has become possible to measure multiple effector functions in a single cell such as cytokine, transcription factors, and cytolytic function. In addition these parameters can be evaluated in conjunction with cellular proliferation. In this chapter we detail these cellular based assays and the methods used to characterize and quantify both phenotype and function of CTL.