Alternative respiration pathway is involved in the response of highland barley to salt stress
Alternative respiration pathway is involved in the response of highland barley to salt stress
复制标题
替代呼吸途径参与青稞对盐胁迫的响应
DOI：
10.1007/s00299-018-2366-6
复制
发表时间：
2019-03-01
影响因子：
6.2
通讯作者：
Bi, Yurong
中科院分区：
文献类型：
--
作者：
He, Qiang;Wang, Xiaomin;Bi, Yurong
Key messageAlternative respiration pathway is involved in the response of highland barley to salt stress.AbstractThe response of two barley seedlings to salt stress was investigated. Results showed that the growth of highland barley (Kunlun 14) and barley (Ganpi 6) had no obvious difference under low concentrations (50 100 and 200mM) of NaCl treatment. However high concentrations of NaCl treatment (300 and 400mM) severely affected the growth of two barley cultivars. Under 300mM NaCl treatment the fresh weight relative water content (RWC) pigments and K+ content reduced more in Ganpi 6 than in Kunlun 14. In contrast the electrolyte leakage and the content of MDA Na+ H2O2 and O-2 increased more in Ganpi 6 than in Kunlun 14. The gene expression of AOX1a HvNHX1 HvNHX3 HvHVP1 HvHVA H(+)ATPase the alternative respiration capacity (V-alt) and the enzymatic activity of SOD POD CAT APX and H(+)ATPase increased more in Kunlun14 than in Ganpi6 under 300mM NaCl treatment whereas the cytochrome respiration capacity (V-cyt) decreased similarly in both barley cultivars. Western blot analysis showed that the protein level of the alternative oxidase (AOX) increased more in Kunlun 14 than in Ganpi 6 under 300mM NaCl treatment. Inhibition of the alternative respiration by salicylhydroxamic acid (SHAM) decreased the fresh weight K+ content V-alt H(+)ATPase activity and the gene expression of AOX1a HvNHX1 HvNHX3 HvHVP1 HvHVA H(+)ATPase but increased the electrolyte leakage MDA and Na+ content in both cultivars under 300mM NaCl treatment. In short alternative respiration is involved in the tolerance of highland barley to salt stress.
