Functional genomic characterization of delipidation elicited by trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) in a polygenic obese line of mice
Functional genomic characterization of delipidation elicited by trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) in a polygenic obese line of mice
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DOI：
10.1152/physiolgenomics.00244.2004
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发表时间：
2005-05-11
影响因子：
4.6
通讯作者：
Odle, J
中科院分区：
文献类型：
--
作者：
House, RL;Cassady, JP;Odle, J
在t10c12- cla诱导的多基因肥胖小鼠体脂减少过程中，测量了基因表达。成年小鼠（n = 185）被分配到2 × 2因子实验中，其中包括非肥胖（icr控制）或肥胖（m16选择）小鼠，喂食含有1%亚油酸（LA）或1% t10c12-CLA的7%脂肪纯化饲料。cla组小鼠第14天体重（BW）较la组降低12% （P < 0.0001）。在第14天，t10c12-CLA使两种动物的附睾、肠系膜和棕色脂肪组织重量占体重的比例分别降低了30%、27%和58%，使肝脏重量/体重增加了34% （P < 0.0001）。从肥胖小鼠的附睾脂肪（第5天和第14天）中分离和汇总总RNA（每个组织每天4个），使用代表bbb20,000个基因的Agilent小鼠寡核苷酸微阵列载片分析基因表达谱。在附睾脂肪（第5天和第14天）中差异表达2倍以上的基因数分别为29个和125个。结果表明，在脂肪组织中，CLA增加了解偶联蛋白（1和2）、肉碱棕榈酰转移酶系统、肿瘤坏死因子- α和caspase-3的表达（P < 0.05），降低了过氧化物酶体增殖激活受体- γ、葡萄糖转运蛋白-4、perilipin、caveolin-1、脂联素、抵抗素和Bcl-2的表达（P < 0.01）。总之，本实验揭示的候选基因将有助于阐明脂肪脱脂的机制。
Gene expression was measured during t10c12-CLA-induced body fat reduction in a polygenic obese line of mice. Adult mice (n = 185) were allotted to a 2 x 2 factorial experiment consisting of either nonobese (ICR-control) or obese (M16-selected) mice fed a 7% fat, purified diet containing either 1% linoleic acid (LA) or 1% t10c12-CLA. Body weight (BW) by day 14 was 12% lower in CLA-compared with LA-fed mice ( P < 0.0001). By day 14, t10c12-CLA reduced weights of epididymal, mesenteric, and brown adipose tissues, as a percentage of BW, in both lines by 30, 27, and 58%, respectively, and increased liver weight/BW by 34% ( P < 0.0001). Total RNA was isolated and pooled (4 pools per tissue per day) from epididymal adipose (days 5 and 14) of the obese mice to analyze gene expression profiles using Agilent mouse oligo microarray slides representing > 20,000 genes. Numbers of genes differentially expressed by greater than or equal to twofold in epididymal adipose (days 5 and 14) were 29 and 125, respectively. It was concluded that, in adipose tissue, CLA increased expression of uncoupling proteins (1 and 2), carnitine palmitoyltransferase system, tumor necrosis factor-alpha (P < 0.05), and caspase-3 but decreased expression of peroxisome proliferator-activated receptor-gamma, glucose transporter-4, perilipin, caveolin-1, adiponectin, resistin, and Bcl-2 (P < 0.01). In conclusion, this experiment has revealed candidate genes that will be useful in elucidating mechanisms of adipose delipidation.