The effect of receptor clustering on vesicle - Vesicle adhesion
The effect of receptor clustering on vesicle - Vesicle adhesion
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DOI：
10.1021/ja0657612
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发表时间：
2006-11-15
影响因子：
15
通讯作者：
Webb, Simon J.
中科院分区：
文献类型：
--
作者：
Mart, Robert J.;Liem, Kwan Ping;Webb, Simon J.
作为我们研究细胞粘附分子在脂筏中的定位如何影响细胞粘附的一部分，我们开发了Cu（1），一种合成的铜（亚氨基二乙酸）帽受体，能够与流体磷脂双层相分离。Cu（1）在囊泡表面聚集成粘附片的程度可以通过改变囊泡的组成来控制。广泛的受体相分离显着增强囊泡-囊泡粘附;只有具有粘附补丁的囊泡（蓝色荧光）粘附到其缀合物组氨酸包被的囊泡（红色荧光），形成大的囊泡聚集体（如图所示）。
As part of our studies into how the localization of cell adhesion molecules into lipid rafts may affect cell adhesion, we developed Cu(1), a synthetic copper(iminodiacetate)-capped receptor able to phase separate from fluid phospholipid bilayers. The extent to which Cu(1) clustered into adhesive patches on the surface of vesicles could be controlled by changing vesicle composition. Extensive receptor phase separation significantly enhanced vesicle−vesicle adhesion; only vesicles with adhesive patches (blue fluorescence) adhered to their conjugate histidine-coated vesicles (red fluorescence) to form large vesicle aggregates (shown).