Comparative Genomic Characterization of Actinobacillus pleuropneumoniae
Comparative Genomic Characterization of Actinobacillus pleuropneumoniae
复制标题
胸膜肺炎放线杆菌的比较基因组特征
DOI：
10.1128/jb.00535-10
复制
发表时间：
2010-11-01
影响因子：
3.2
通讯作者：
Zhou, Rui
中科院分区：
文献类型：
--
作者：
Xu, Zhuofei;Chen, Xiabing;Zhou, Rui
摘要猪传染性胸膜肺炎是一种严重危害养猪业的致死性呼吸道传染病，其病原菌为革兰氏阴性杆菌胸膜肺炎。为了更好地解释血清型多样性的遗传背景，采用快速高通量方法对胸膜肺炎弧菌1、2、4、6、9、10、11、12和13号血清型的9株参考菌株的基因组进行了测序。基于相应血清型参考菌株的12个基因组，包括该细菌的三个公开可获得的完整基因组(血清型3、5b和7)，我们进行了全面的比较基因组学分析，并首次报道了该病原菌的全球基因组特征。对26,012个预测蛋白编码基因的聚类分析表明，胸膜肺炎弧菌泛基因组由3,303个基因簇组成，包含1,709个核心基因组基因、822个分布基因和772个毒株特异性基因。比较了衣壳多糖和脂多糖O抗原在生物发生过程中所涉及的基因组成分与血清型多样性的关系，描绘了它们的遗传多样性。我们的发现进一步揭示了与胸膜肺炎弧菌血清型多样性相关的基因组特征，并为该病的发病机制研究和临床/流行病学应用提供了更广泛的见解。
ABSTRACT The Gram-negative bacterium Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumoniae, a lethal respiratory infectious disease causing great economic losses in the swine industry worldwide. In order to better interpret the genetic background of serotypic diversity, nine genomes of A. pleuropneumoniae reference strains of serovars 1, 2, 4, 6, 9, 10, 11, 12, and 13 were sequenced by using rapid high-throughput approach. Based on 12 genomes of corresponding serovar reference strains including three publicly available complete genomes (serovars 3, 5b, and 7) of this bacterium, we performed a comprehensive analysis of comparative genomics and first reported a global genomic characterization for this pathogen. Clustering of 26,012 predicted protein-coding genes showed that the pan genome of A. pleuropneumoniae consists of 3,303 gene clusters, which contain 1,709 core genome genes, 822 distributed genes, and 772 strain-specific genes. The genome components involved in the biogenesis of capsular polysaccharide and lipopolysaccharide O antigen relative to serovar diversity were compared, and their genetic diversity was depicted. Our findings shed more light on genomic features associated with serovar diversity of A. pleuropneumoniae and provide broader insight into both pathogenesis research and clinical/epidemiological application against the severe disease caused by this swine pathogen.