Enhanced biodegradation of phenanthrene in oil tar-contaminated soils supplemented with Phanerochaete chrysosporium
Enhanced biodegradation of phenanthrene in oil tar-contaminated soils supplemented with Phanerochaete chrysosporium
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DOI：
10.1128/aem.58.9.3117-3121.1992
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发表时间：
1992-09
影响因子：
4.4
通讯作者：
T. Brodkorb;R. Legge
中科院分区：
文献类型：
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作者：
T. Brodkorb;R. Legge
近年来，白色腐真菌黄孢原毛平革菌已显示出作为适合于分解包括多环芳烃（PAH）在内的广谱环境污染物的生物体的前景。本研究的重点是确定是否P. chrysosporium可以有效地在实际的现场样品的石油焦油污染的土壤。土壤中负载[14 C]菲作为代表多环芳烃的模型化合物。在具有缓冲（pH 5.0至5.5）的静态有氧条件下存在的具有原生植物群的土壤在21天内显示出20%的完全矿化。添加P. chrysosporium是协同的，在21天内完全矿化约38%。除完全矿化外，在存在黄孢原毛平革菌的情况下，放射性标记极性浸提物（可溶性和结合性）的比例也有所增加。从这项研究中，很明显，本地土壤微生物区系可以促使在一些污染的土壤中的多环芳烃的充分矿化，这种矿化可以加强时，补充白色腐真菌P. chrysosporium。通过进一步完善，该系统可能被证明是一种有效的多环芳烃污染土壤的生物修复技术。
In recent years, the white rot fungus Phanerochaete chrysosporium has shown promise as an organism suitable for the breakdown of a broad spectrum of environmental pollutants, including polynuclear aromatic hydrocarbons (PAHs). The focus of this study was to determine whether P. chrysosporium could effectively operate in an actual field sample of oil tar-contaminated soil. The soil was loaded with [14C]phenanthrene to serve as a model compound representative of the PAHs. Soil with the native flora present under static, aerobic conditions with buffering (pH 5.0 to 5.5) displayed full mineralization on the order of 20% in 21 days. The addition of P. chrysosporium was synergistic, with full mineralization on the order of 38% in 21 days. In addition to full mineralization, there was an increase in the proportion of radiolabelled polar extractives, both soluble and bound, in the presence of P. chrysosporium. From this study, it is apparent that the native soil microflora can be prompted into full mineralization of PAHs in some contaminated soils and that this mineralization can be enhanced when supplemented with the white rot fungus P. chrysosporium. With further refinement, this system may prove an effective bioremediation technology for soils contaminated with PAHs.