Expanding the Mot1 subfamily: 89B Helicase encodes a new Drosophila melanogaster SNF2-related protein which binds to multiple sites on polytene chromosomes
Expanding the Mot1 subfamily: 89B Helicase encodes a new Drosophila melanogaster SNF2-related protein which binds to multiple sites on polytene chromosomes
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DOI：
10.1093/nar/24.16.3121
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发表时间：
1996-08-15
影响因子：
14.9
通讯作者：
Zak, NB
中科院分区：
文献类型：
--
作者：
GoldmanLevi, R;Miller, C;Zak, NB
SNF2家族的许多蛋白具有相似的DNA依赖性atp酶/推测解旋酶结构域，参与DNA损伤的全球转录控制和处理。我们在这里报道了89B解旋酶的部分克隆和表征，该基因编码SNF2家族的新成员果蝇。89B解旋酶蛋白在其atp酶/解旋酶结构域与全局转录激活因子SNF2和Brahma以及DNA修复蛋白ERCC6和RAD54高度同源。然而，它与酿酒酵母蛋白Mot1最为惊人地相似，后者是一种转录抑制物，具有许多尚未描述的同源基因。89B解旋酶在果蝇发育过程中表达，其大转录物编码bbb200 kDa蛋白，用抗89B解旋酶抗体染色显示该蛋白在早期胚胎中均匀存在，然后定位于腹侧神经索和大脑。89B解旋酶与数百个随机分布的特异性位点结合，其与Mot1的同源性、其广泛的发育表达以及在幼虫唾液腺细胞多染色体上的大量靶点提示89B解旋酶可能在染色体代谢，特别是全局转录调控中发挥作用。
Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage, We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 family. 89B Helicase protein shows a high degree of homology in its ATPase/helicase domain to the global transcriptional activators SNF2 and Brahma and to the DNA repair proteins ERCC6 and RAD54. It is, however, most strikingly similar to the Saccharomyces cerevisiae protein Mot1, a transcriptional repressor with many target genes for which no homologue has yet been described, 89B helicase is expressed throughout fly development and its large transcript encodes a >200 kDa protein, Staining with anti-89B Helicase antibodies reveals that the protein is present uniformly in early embryos and then becomes localized to the ventral nerve cord and brain, On the polytene chromosomes, 89B Helicase is bound to several hundred specific sites that are randomly distributed, The homology of 89B Helicase to Mot1, its widespread developmental expression and its large number of targets on the polytene chromosomes of larval salivary gland cells suggest that 89B Helicase may play a role in chromosomal metabolism, particularly global transcriptional regulation.