Zyflamend reduces LTB4 formation and prevents oral carcinogenesis in a 7,12-dimethylbenz[alpha]anthracene (DMBA)-induced hamster cheek pouch model.
Zyflamend reduces LTB4 formation and prevents oral carcinogenesis in a 7,12-dimethylbenz[alpha]anthracene (DMBA)-induced hamster cheek pouch model.
复制标题
Zyflamend 在 7,12-二甲基苯并蒽 (DMBA) 诱导的仓鼠颊囊模型中减少 LTB4 形成并预防口腔癌发生。
DOI：
10.1093/carcin/bgn181
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发表时间：
2008
期刊：
影响因子：
4.7
通讯作者：
Newman,RobertA
中科院分区：
文献类型：
--
作者：
Yang,Peiying;Sun,Zheng;Chan,Diana;Cartwright,CarrieA;Vijjeswarapu,Mary;Ding,Jibin;Chen,Xiaoxin;Newman,RobertA
Aberrant arachidonic acid metabolism, especially altered cyclooxygenase and 5-lipoxygenase (LOX) activities, has been associated with chronic inflammation as well as carcinogenesis in human oral cavity tissues. Here, we examined the effect of Zyflamend®, a product containing 10 concentrated herbal extracts, on development of 7,12-dimethylbenz[α]anthracene (DMBA)-induced inflammation and oral squamous cell carcinoma (SCC). A hamster cheek pouch model was used in which 0.5% DMBA was applied topically onto the left cheek pouch of male Syrian golden hamsters either three times per week for 3 weeks (short term) or 6 weeks (long term). Zyflamend was then applied topically at one of three different doses (25, 50 and 100 μl) onto the left cheek pouch three times for 1 week (short-term study) or chronically for 18 weeks. Zyflamend significantly reduced infiltration of inflammatory cells, incidence of hyperplasia and dysplastic lesions, bromodeoxyuridine-labeling index as well as number of SCC in a concentration-dependent manner. Application of Zyflamend (100 μl) reduced formation of leukotriene B4(LTB4) by 50% compared with DMBA-treated tissues. The reduction of LTB4was concentration dependent. The effect of Zyflamend on inhibition of LTB4formation was further confirmed within vitrocell-based assay. Adding LTB4to RBL-1 cells, a rat leukemia cell line expressing high levels of 5-LOX and LTA4hydrolase, partially blocked antiproliferative effect of Zyflamend. This study demonstrates that Zyflamend inhibited LTB4formation and modulated adverse histopathological changes in the DMBA-induced hamster cheek pouch model. The study suggests that Zyflamend might prevent oral carcinogenesis at the post-initiation stage.
