Fast retrospectively gated quantitative four-dimensional (4D) cardiac micro computed tomography imaging of free-breathing mice
Fast retrospectively gated quantitative four-dimensional (4D) cardiac micro computed tomography imaging of free-breathing mice
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DOI：
10.1097/01.rli.0000251572.56139.a3
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发表时间：
2007-02-01
影响因子：
6.7
通讯作者：
Holdsworth, David W.
中科院分区：
文献类型：
--
作者：
Drangova, Maria;Ford, Nancy L.;Holdsworth, David W.
目的：对自由呼吸小鼠进行回顾性门控三维心脏显微计算机断层扫描(CT)。材料和方法：5只C57B16小鼠使用带有滑环的平板探测器的锥形束扫描仪进行扫描。在注射血管内碘化造影剂后，在50秒内获得投影图像，同时扫描仪旋转10个完整的旋转。在图像采集的同时记录小鼠的呼吸和心电信号。结果：所有小鼠心脏动态3D图像均以12毫秒间隔重建，体素间距各向同性。平均射血分数为58.2±-4.6%，平均心输出量为11.4+/-1.3毫升/分。整个扫描测量的入射剂量为28cGy.对相同动物的重复扫描显示，对象内的变异性小于对象间的变异性。结论：我们开发了一种高分辨率的微型计算机断层扫描方法，可以在获取时间小于1分钟的情况下评估自由呼吸小鼠的心功能和形态。
Objective: We sought to demonstrate retrospectively gated dynamic 3D cardiac micro computed tomography (CT) of free-breathing mice.Materials and Methods: Five C57B16 mice were scanned using a cone-beam scanner with a slip-ring-mounted flat-panel detector. After the injection of an intravascular iodinated contrast agent, projection images were acquired over the course of 50 seconds, while the scanner rotated through 10 complete rotations. The mouse respiratory and electrocardiogram signals were recorder simultaneously with image acquisition. After acquisition, the projection images were retrospectively sorted into projections belonging to different cardiac time points, occurring only during expiration.Results: Dynamic 3D cardiac images, with isotropic 150-mu m voxel spacing, were reconstructed at 12-millisecond intervals throughout the cardiac cycle in all mice. The average ejection fraction and cardiac output were 58.2 +/- 4.6% and 11.4 +/- 1.3 mL/min, respectively. The measured entrance dose for the entire scan was 28 cGy. Repeat scans of the same animals showed that intrasubject variability was smaller than intersubject variability.Conclusions: We have developed a high-resolution micro computed tomography method for evaluating the cardiac function and morphology of free-breathing mice in acquisition times shorter than 1 minute.