In vitro evaluation of random and aligned polycaprolactone/gelatin fibers via electrospinning for bone tissue engineering
In vitro evaluation of random and aligned polycaprolactone/gelatin fibers via electrospinning for bone tissue engineering
复制标题
通过静电纺丝对骨组织工程随机和排列聚己内酯/明胶纤维进行体外评估
DOI：
10.1080/09205063.2015.1065598
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发表时间：
2015-01-01
影响因子：
3.6
通讯作者：
Li, Lihua
中科院分区：
文献类型：
--
作者：
Guo, Zhenzhao;Xu, Jiaming;Li, Lihua
支架作为组织工程的基本要素，应提供适当的化学和结构线索，以指导组织再生。本研究采用静电纺丝法制备了不同质量比的聚己内酯（PCL）/明胶纤维支架。通过FTIR和拉伸测试对PCL/明胶纤维支架的化学、结构和力学性能进行了表征。各组平均直径在334.96 ± 41.43 nm ~ 363.78 ± 50.49 nm之间。PCL与明胶共混可提高支架的力学性能。细胞培养结果表明，聚己内酯与明胶的质量比对细胞的生长行为无明显影响，而纤维的取向对细胞的生长行为有一定的影响。在排列的纤维上培养的细胞具有更高的伸长比、更强的细胞增殖和更高的碱性磷酸酶活性。本研究为骨组织工程支架材料的设计和制备提供了有益的参考。
Scaffold, as an essential element of tissue engineering, should provide proper chemical and structural cues to direct tissue regeneration. In this study, aligned and random polycaprolactone (PCL)/gelatin fibrous scaffolds with different mass ratio were electrospun. Chemical, structural, and mechanical properties of PCL/gelatin fibrous scaffolds were characterized by FTIR and tensile measurements. The average diameters of different groups were between 334.96 ± 41.43 nm and 363.78 ± 50.49 nm. Blending PCL with gelatin increased the mechanical properties of the scaffolds. The cell culture results demonstrated that the mass ratio of PCL and gelatin showed no obvious effects on cell behavior, whereas the cell growth behavior was affected by the fibers orientation. Higher elongation ratio, enhanced cell proliferation and elevated alkaline phosphatase activity were observed for cells cultured on aligned fibers. The findings in our research provide insightful information for the design and fabrication of scaffolds for bone tissue engineering.