Genetic diversity of coxsackievirus A16 associated with hand, foot, and mouth disease epidemics in Japan from 1983 to 2003
Genetic diversity of coxsackievirus A16 associated with hand, foot, and mouth disease epidemics in Japan from 1983 to 2003
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DOI：
10.1128/jcm.00718-06
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发表时间：
2007-01-01
影响因子：
9.4
通讯作者：
Suzuki, Hitoshi
中科院分区：
文献类型：
--
作者：
Hosoya, Mitsuaki;Kawasaki, Yukihiko;Suzuki, Hitoshi
为了阐明限制区域内与手足口病（HFMD）流行相关的柯萨奇病毒A16（CV-A16）毒株的时间遗传多样性及其与其他地区分离毒株的遗传关系，我们调查了1983 - 2003年日本福岛与HFMD流行相关的129株CV-A16毒株的遗传多样性，根据VP 4基因序列进行系统发育分析，比较其与日本其他地区和中国分离的49株CV-A16毒株的亲缘关系。从1983年至2003年在福岛分离的CV-A16菌株的系统发育重建表明，与1984年至1994年发生的手足口病流行相关的三个不同的遗传差异簇1987 - 1998年手足口病疫情（包括1985年和1991年疫情）（包括1988年和1998年爆发）和1995年至2003年手足口病疫情（包括1995年和2002年爆发）。在福岛每个时期分离的CV-A16菌株与在日本其他地区和中国基本相同的时间段分离的菌株形成一个单一的集群。我们的研究结果表明，在日本福岛引起手足口病流行的CV-A16毒株在21次流行中发生了两次基因变化，在其他地区引起手足口病流行的CV-A16毒株中也观察到了变化。我们的结论是，CV-A16相关的手足口病在日本的反复爆发，部分原因是引入了基因改变的CV-A16菌株，可能会传播到海外。
To clarify the chronologic genetic diversity of coxsackievirus A16 (CV-A16) strains associated with hand, foot, and mouth disease (HFMD) epidemics in a restricted area and their genetic relation with those isolated in other areas, we investigated the genetic diversity of the 129 CV-A16 strains associated with HFMD epidemics in Fukushima, Japan, from 1983 to 2003, and compared their genetic relation to 49 CV-A16 strains isolated in other areas of Japan and in China by using phylogenetic analysis based on the VP4 sequences. Phylogenetic reconstruction of the CV-A16 strains isolated in Fukushima from 1983 to 2003 demonstrated three distinct genetically divergent clusters related to HFMD epidemics that occurred from 1984 to 1994 (including the 1985 and 1991 outbreaks), HFMD epidemics from 1987 to 1998 (including the 1988 and 1998 outbreaks), and HFMD epidemics from 1995 to 2003 (including the 1995 and 2002 outbreaks). CV-A16 strains isolated during each period in Fukushima formed a single cluster with those isolated during essentially the same time period in other areas of Japan and in China. Our results demonstrated that prevalent CV-A16 strains causing HFMD in Fukushima, Japan, genetically changed twice during 21 epidemics, and changes were also observed in the CV-A16 strains causing HFMD epidemics in other areas. We concluded that repeated outbreaks of CV-A16-related HFMD in Japan were caused, in part, by the introduction of genetically changed CV-A16 strains, which might be transmitted overseas.