Typing of Listeria monocytogenes Strains by Repetitive Element Sequence-Based PCR
Typing of Listeria monocytogenes Strains by Repetitive Element Sequence-Based PCR
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DOI：
10.1128/jcm.37.1.103-109.1999
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发表时间：
1999-01
影响因子：
9.4
通讯作者：
Barbara Jeršek;P. Gilot;M. Gubina;N. Klun;J. Mehle;E. Tcherneva;N. Rijpens;L. Herman
中科院分区：
文献类型：
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作者：
Barbara Jeršek;P. Gilot;M. Gubina;N. Klun;J. Mehle;E. Tcherneva;N. Rijpens;L. Herman
摘要 单核细胞增生李斯特菌菌株具有短重复基因外回文 (REP) 元件和肠杆菌重复基因间共有 (ERIC) 序列。我们使用基于重复元件序列的 PCR (rep-PCR) 来评估 REP 和 ERIC 元件对从人类、动物和食品中分离出的单核细胞增生李斯特菌菌株进行分型的潜力。根据rep-PCR指纹图谱，单增李斯特菌菌株被分为与分离来源相匹配的四个主要簇。人类和动物分离物的rep-PCR指纹与食品分离物的不同。 rep-PCR 指纹的计算机评估允许区分每个主要簇内的测试血清型 1/2a、1/2b、1/2c、3b 和 4b。对于 REP-和 ERIC-PCR，针对 52 个流行病学无关的单增李斯特菌分离株计算出的辨别指数为 0.98。我们的结果表明，rep-PCR 可以为单核细胞增生李斯特菌分型提供替代方法。
ABSTRACT Listeria monocytogenes strains possess short repetitive extragenic palindromic (REP) elements and enterobacterial repetitive intergenic consensus (ERIC) sequences. We used repetitive element sequence-based PCR (rep-PCR) to evaluate the potential of REP and ERIC elements for typing L. monocytogenes strains isolated from humans, animals, and foods. On the basis of rep-PCR fingerprints, L. monocytogenes strains were divided into four major clusters matching origin of isolation. rep-PCR fingerprints of human and animal isolates were different from those of food isolates. Computer evaluation of rep-PCR fingerprints allowed discrimination among the tested serotypes 1/2a, 1/2b, 1/2c, 3b, and 4b within each major cluster. The index of discrimination calculated for 52 epidemiologically unrelated isolates of L. monocytogeneswas 0.98 for REP- and ERIC-PCR. Our results suggest that rep-PCR can provide an alternative method for L. monocytogenes typing.