What does the difference between the female and male gametophytes of Saccharina japonica remind us of?
What does the difference between the female and male gametophytes of Saccharina japonica remind us of?
复制标题
甘蔗雌雄配子体的区别提醒我们什么
DOI：
10.1127/1864-1318/2014/0145
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Zhou, Zhi-Gang
中科院分区：
文献类型：
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作者：
Bi, Yan-Hui;Zhou, Zhi-Gang
本文报道了日本海带（Aresch.）C.E.莱恩角Mayes，Druehl et G.W. Saunders，减数分裂后产生孢子，孢子发育成性比为1：1的单倍体雌、雄配子体。这一比例以及由孤雌生殖孢子体产生的游动孢子所产生的配子体全部为雌性的结果表明，S.日本的。为了了解雌、雄配子体的分子差异，构建了抑制消减cDNA文库。结果表明，lhcf6是海带雌、雄配子体差异表达基因之一，这种差异表达可能是由不同的启动子引起的。虽然没有性别特异性基因进行筛选，一些性别相关的分子标记被开发出来。在多酶预处理制备的高质量染色体的基础上，将两个与雌配子体有关的分子标记共定位在孢子体染色体上，表明它们是雌染色体特异的细胞遗传学DNA标记。S.利用荧光原位杂交技术，结合端粒、着丝粒等重复序列，对海带孢子体染色体进行配对，可以解决海带具有性染色体的问题。
The diploid sporophytes of brown seaweed, Saccharina japonica (Aresch.) C.E. Lane, C. Mayes, Druehl et G.W. Saunders, produce spores after meiosis, which develop into haploid female and male gametophytes with a sex ratio of 1:1. This ratio, as well as the results that all gametophytes produced by zoospores generated by parthenogenetic sporophytes are females, suggest that an X/Y-like sex-determination system exists in S. japonica. To understand the molecular differences between female and male gametophytes, a suppression subtractive cDNA library was constructed. It was found that lhcf6 was one of the differentially expressed genes, and that this differential transcription between the kelp female gametophytes and male ones might result from different promoters. Although no sex-specific genes were screened, several sex-related molecular markers were developed. Based on the high quality chromosomes prepared by pretreatment with multi-enzymes, two molecular markers in relation to the female gametophytes were co-localized on the sporophyte chromosomes, indicating they are female chromosome-specific cytogenetic DNA markers. Whether S. japonica possesses a sex chromosome might be resolved by pairing the kelp sporophyte chromosomes with the help of localized repetitive sequences such as telomere and centromere using fluorescence in situ hybridization techniques.