Extracellular matrix (mesoglea) of Hydra vulgaris. II. Influence of collagen and proteoglycan components on head regeneration.
Extracellular matrix (mesoglea) of Hydra vulgaris. II. Influence of collagen and proteoglycan components on head regeneration.
复制标题
寻常水螅的细胞外基质（mesoglea）。
DOI：
10.1016/0012-1606(91)90267-7
复制
发表时间：
1991
影响因子：
2.7
通讯作者：
Zhang,XM
中科院分区：
文献类型：
--
作者：
SarrasJr,MP;Meador,D;Zhang,XM
水螅的特征是它们的体壁组织成上皮双层，中间有一层称为中胶层的非细胞层。作为先前研究的延伸，中胶层是一种原始的基底膜，保留了间质细胞外基质的一些特征，本研究旨在分析中胶层组分在水螅头部再生过程中的作用。研究中使用了影响胶原蛋白加工或胶原蛋白二级结构的药物（β-氨基丙腈、2，2 ′-联吡啶和顺式-4-羟基-1-脯氨酸）和抑制糖胺聚糖链添加至蛋白聚糖核心蛋白的药物（对硝基苯基-β-d-吡喃木糖苷）。这些研究表明，胶原蛋白或蛋白聚糖的结构的变化引起的头部再生的水螅堵塞监测超过48小时的时间。一旦药物被移除，头部再生的阻断是可逆的，表明药物对生物体没有一般毒性作用。放射性示踪剂研究还表明，头部再生的阻断不仅仅是由于药物对蛋白质合成的普遍抑制。各种对照表明，在这些研究中使用的条件下，每种药物都会影响中胶层组分。这些观察结果表明，保存正常的mesoglea结构所需的水螅头再生进行。
Hydra are characterized by having their body wall organized as an epithelial bilayer with an intervening acellular layer termed the mesoglea. As an extension of the previous study which indicated that mesoglea is a primitive basement membrane which has retained some characteristics of interstitial extracellular matrix, the present study was undertaken to analyze the role of mesoglea components during head regeneration inHydra vulgaris. Studies were conducted that utilized drugs that affect collagen processing or secondary collagen structure (β-aminoproprionitrile; 2,2′-dipydridyl; andcis-4-hydroxy-l-proline) and a drug that inhibits addition of glycosaminoglycan chains to proteoglycan core proteins (p-nitrophenyl-β-d-xylopyranoside). These studies indicated that alternations in the structure of collagens or proteoglycans caused blockage of head regeneration in Hydra as monitored over a 48-hr period. Blockage of head regeneration was reversible once the drugs were removed, indicating that the drugs were not having a general toxic effect on the organism. Radiotracer studies also indicated that blockage of head regeneration was not simply due to a general depression of protein synthesis by the drugs. Various controls indicated that each drug was affecting mesoglea components under the conditions utilized in these studies. These observations indicate that preservation of normal mesoglea structure is required for Hydra head regeneration to proceed.