Anaerobic Ammonium Oxidation Bacterial Communities in Long-term Cultivated Sludge : A Comparison between Mesophilic and Psychrophilic Conditions
Anaerobic Ammonium Oxidation Bacterial Communities in Long-term Cultivated Sludge : A Comparison between Mesophilic and Psychrophilic Conditions
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DOI：
10.2521/jswtb.52.1
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发表时间：
2016
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影响因子：
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通讯作者：
T. Kamei;S. Shimizu;Yasuhiro Tanaka;F. Kazama
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作者：
T. Kamei;S. Shimizu;Yasuhiro Tanaka;F. Kazama
厌氧氨氧化(Anammox)是一种很有前途的废水脱氮方法。我们的目标是比较在中温(35℃)和嗜冷(15℃)条件下培养的淡水环境污泥(河流、湖泊和大坝水库)样本中的厌氧氨氧化细菌。在中温条件下，培养400d后，脱氮率(NRR)有所提高，而在嗜冷条件下，脱氮率没有显著提高。在附加培养超过400天后，NRRS值增加到几乎与中温条件下相同的水平。系统发育分析表明，从6个Anammox反应器样品中可划分出4个Anammox克隆系统型，且多数系统型在多个样品间共享。中温样品与卡氏假丝酵母菌KSU-1和中华假丝酵母菌JPN1的亲缘关系分别为99%和100%，而嗜冷性样品的亲缘关系分别为94%和96%。分别为40。培养温度影响厌氧氨氧化菌种的丰度。此外，嗜冷样品的亲缘关系被归入Brocadia谱系的嗜冷厌氨氧化类群，表明存在适应低温的厌氨氧化细菌。
Anaerobic ammonium oxidation (anammox) is a promising option for removing nitrogen from wastewater. We aimed to compare anammox bacteria from samples of freshwater environmental sludge (river, lake, and dam reservoir) cultivated under mesophilic (35 ℃ ) and psychrophilic (15 ℃ ) conditions. An increase in nitrogen removal rate (NRR) was found in the mesophilic condition after 400 days cultivation, whereas no significant increment was detected in the psychrophilic condition. After additional cultivation for over 400 days, NRRs values increased to almost same levels of those of mesophilic condition. Phylogenetic analysis showed that 4 anammox clonal phylotypes could be categorized from 6 samples from anammox reactors, and most phylotypes were shared among multiple samples. Phylotypes of the mesophilic samples had 99% and 100% identity with Candidatus Jettenia caeni strain KSU － 1 and Candidatus Brocadia sinica strain JPN1, respectively, whereas phylotypes of psychrophilic samples showed 94% and 96% identity with Candidatus Brocadia fulgida and Candidatus Brocadia sp. 40, respectively. Cultivation temperature affected enriched anammox species. Furthermore, phylotypes of psychrophilic samples were categorized into psychrophilic anammox group of Brocadia lineage, indicating the existence of low temperature-adapting anammox bacteria.