Reorganization of chromatin is an early response to nitrogen starvation in Schizosaccharomyces pombe
Reorganization of chromatin is an early response to nitrogen starvation in Schizosaccharomyces pombe
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DOI：
10.1007/s00412-008-0180-6
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发表时间：
2009-02-01
期刊：
影响因子：
1.6
通讯作者：
Bjerling, Pernilla
中科院分区：
文献类型：
--
作者：
Alfredsson-Timmins, Jenny;Kristell, Carolina;Bjerling, Pernilla
有几个记录在案的基因激活过程中亚核定位发生变化的事件。然而，关于核外围是否是基因抑制或激活的区室以及基因在基因激活过程中是否移动到核膜（NM）的孔中，存在相互矛盾的数据。裂殖酵母的氮饥饿是研究基因诱导的良好模型系统，因为它会导致数百个基因的快速调节。在这项研究中，研究了氮抑制的两个基因簇的亚核定位。在正常生长条件下，与纺锤体极体相比，基因簇定位于细胞核相对侧的核外围。这种受限定位依赖于组蛋白脱乙酰酶 Clr3，已知该酶可以转录抑制这些簇中的基因。氮耗尽后 20 分钟，观察到两个基因座的亚核定位发生了巨大变化，从 NM 转向核内部。至少对于其中一个簇来说，这种运动显然是依赖于转录的。本文提供的数据说明了基因激活和核重组事件如何相互关联，并提供了如何维持核组织的建议。
There are several documented events of changes in subnuclear localization during gene activation. However, there are conflicting data on whether the nuclear periphery is a compartment for gene repression or activation and whether genes are moved to the pores at the nuclear membrane (NM) or not during gene activation. Nitrogen starvation of fission yeast serves as a good model system for studying gene induction, as it causes fast regulation of hundreds of genes. In this study, the subnuclear localization of two gene clusters repressed by nitrogen was investigated. During normal growth conditions, the gene clusters localized to the nuclear periphery at the opposite side of the nucleus as compared to the spindle pole body. This constrained localization was dependent on the histone deacetylase Clr3, known to transcriptionally repress genes in these clusters. Already 20 min after nitrogen depletion, drastic changes in subnuclear localization of the two loci were observed, away from the NM toward the nuclear interior. At least for one of the clusters, the movement was clearly transcription dependent. Data presented in this paper illustrates how interconnected events of gene activation and nuclear reorganization are as well as provides a suggestion of how nuclear organization might be maintained.