D-JNK signaling in visceral muscle cells controls the laterality of the Drosophila gut.
D-JNK signaling in visceral muscle cells controls the laterality of the Drosophila gut.
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DOI：
10.1016/j.ydbio.2007.08.048
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发表时间：
2007-11
影响因子：
2.7
通讯作者：
Kiichiro Taniguchi;Shunya Hozumi;Reo Maeda;Masashi Ooike;Takeshi Sasamura;T. Aigaki;K. Matsuno
中科院分区：
文献类型：
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作者：
Kiichiro Taniguchi;Shunya Hozumi;Reo Maeda;Masashi Ooike;Takeshi Sasamura;T. Aigaki;K. Matsuno
虽然从外部看，双侧动物似乎具有左右对称，但它们的内部器官往往表现出方向性和刻板印象的左-右对称。在脊椎动物中建立LR轴的机制已经被广泛研究。然而，每个器官如何形成其相对于LR轴的LR不对称形态仍然不清楚。在这里，我们证明了果蝇Jun氨基末端激酶(D-JNK)信号参与了果蝇前-中肠(AMG)的LR不对称环路。编码D-JNK磷酸酶的PUC突变胚胎表现出AMG的随机偏侧性。AMG的定向LR环需要在躯干内脏中胚层被PUC下调D-JNK信号。LR不对称环不仅需要下调，还需要激活D-JNK信号。我们还发现，环状内脏肌(CVM)的LR不对称细胞重排受D-JNK信号的调节，是AMG的LR不对称循环所必需的。Rho家族的小GTP酶rac1在此过程中增强了D-JNK信号。我们的结果还表明，引发LR不对称肠环的基本机制可能在脊椎动物和无脊椎动物之间是保守的。
Although bilateral animals appear to have left–right (LR) symmetry from the outside, their internal organs often show directional and stereotypical LR asymmetry. The mechanisms by which the LR axis is established in vertebrates have been extensively studied. However, how each organ develops its LR asymmetric morphology with respect to the LR axis is still unclear. Here, we showed that Drosophila Jun N-terminal kinase (D-JNK) signaling is involved in the LR asymmetric looping of the anterior-midgut (AMG) in Drosophila. Mutant embryos of puckered (puc), which encodes a D-JNK phosphatase, showed random laterality of the AMG. Directional LR looping of the AMG required D-JNK signaling to be down-regulated by puc in the trunk visceral mesoderm. Not only the down-regulation, but also the activation of D-JNK signaling was required for the LR asymmetric looping. We also found that the LR asymmetric cell rearrangement in the circular visceral muscle (CVM) was regulated by D-JNK signaling and required for the LR asymmetric looping of the AMG. Rac1, a Rho family small GTPase, augmented D-JNK signaling in this process. Our results also suggest that a basic mechanism for eliciting LR asymmetric gut looping may be conserved between vertebrates and invertebrates.