Genomic organization of the gene coding for human pre-B-cell colony enhancing factor and expression in human fetal membranes
Genomic organization of the gene coding for human pre-B-cell colony enhancing factor and expression in human fetal membranes
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DOI：
10.1677/jme.0.0260107
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发表时间：
2001-04-01
影响因子：
3.5
通讯作者：
Bryant-Greenwood, GD
中科院分区：
文献类型：
--
作者：
Ognjanovic, S;Bao, S;Bryant-Greenwood, GD
前b细胞集落增强因子（PBEF）首次从活化的外周血淋巴细胞cDNA文库中分离到，并被发现参与b细胞前体的成熟。它随后被确定为在体外通过扩张人胎膜而上调的基因之一。本文报道了该基因的基因组组织，该基因由11个外显子和10个内含子组成，跨越34.7 kb的基因组DNA。在任何现有的数据库中，该基因和蛋白质都与其他细胞因子没有任何同源性。使用两个启动子（近端和远端）可能导致PBEF转录本的差异，组织特异性表达。5'侧区缺乏将其与造血细胞因子置于一起的经典序列基序；然而，它有几个假定的调控元件，这表明该基因可能在化学和机械上对转录诱导剂有反应。三种PBEF mRNA转录本在正常和感染的人胎膜中均被观察到，但显著上调(P
Pre-B-cell colony enhancing factor (PBEF) was first isolated from an activated peripheral blood lymphocyte cDNA library and was found to be involved in the maturation of B-cell precursors. It was subsequently identified as one of the genes upregulated by distending the human fetal membranes in vitro. Here we report on the genomic organization of this gene, which is composed of 11 exons and 10 introns, spanning 34.7 kb of genomic DNA. Neither the gene nor the protein has any homology with other cytokines in any currently available database. The use of two promoters (proximal and distal) may result in differential, tissue specific expression of the PBEF transcripts. The 5'-flanking region lacks the classical sequence motif that would place it with the hematopoietic cytokines; however, it has several putative regulatory elements, suggesting that this gene may be chemically and mechanically responsive to inducers of transcription.The three PBEF mRNA transcripts were observed in both normal and infected human fetal membranes but were significantly upregulated (P