Roles of capsaicin-sensitive sensory nerves, endogenous nitric oxide, sulfhydryls, and prostaglandins in gastroprotection by momordin Ic, an oleanolic acid oligoglycoside, on ethanol-induced gastric mucosal lesions in rats
Roles of capsaicin-sensitive sensory nerves, endogenous nitric oxide, sulfhydryls, and prostaglandins in gastroprotection by momordin Ic, an oleanolic acid oligoglycoside, on ethanol-induced gastric mucosal lesions in rats
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DOI：
10.1016/s0024-3205(99)00241-6
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发表时间：
1999-06-04
期刊：
影响因子：
6.1
通讯作者：
Yoshikawa, M
中科院分区：
文献类型：
--
作者：
Matsuda, H;Li, YH;Yoshikawa, M
辣椒素敏感感觉神经（CPSN）、内源性一氧化氮（NO）、巯基（SHs）、前列腺素（pg）在苦藤果中齐墩果酸寡糖苷辣素Ic保护胃中的作用SCHRAD。研究了乙醇对大鼠胃粘膜损伤的影响。苦瓜素Ic （10 mg/kg, p.o.）可能抑制乙醇诱导的胃粘膜病变。辣椒素（125 mg/kg，共溶CPSN）、n - g -硝基- l -精氨酸甲酯（L-NAME, 70 mg/kg，共溶NO合成酶抑制剂）、n -乙基马来酰亚胺NEM， 10 mg/kg，共溶SHs抑制剂)或吲哚美辛（10 mg/kg，共溶PGs抑制剂）预处理后，苦参素Ic的作用明显减弱。L-NAME的衰减被NO合成酶底物l -精氨酸（300 mg/kg，静脉注射）消除，而不被d -精氨酸（300 mg/kg，静脉注射，l -精氨酸的对映体）消除。辣椒素与吲哚美辛、NEM或L-NAME联合使用的效果并不比辣椒素单独使用更有效。吲哚美辛与NEM、吲哚美辛与L-NAME或吲哚美辛与NEM、L-NAME单用均能增加其衰减。提示CPSN对大鼠胃粘膜损伤具有重要的保护作用，内源性pg、NO和SHs相互作用。(C) 1999 Elsevier Science Inc.；
The roles of capsaicin-sensitive sensory nerves (CPSN), endogenous nitric oxide (NO), sulfhydryls (SHs), prostaglandins (PGs) in the gastroprotection by momordin Ic, an oleanolic acid oligoglycoside isolated from the fruit of Kochia scoparia (L.) SCHRAD., on ethanol-induced gastric mucosal lesions were investigated in rats. Momordin Ic (10 mg/kg, p.o.) potentially inhibited ethanol-induced gastric mucosal lesions. The effect of momordin Ic was markedly attenuated by the pretreatment with capsaicin (125 mg/kg in total, s.c., an ablater of CPSN), N-G-nitro-L-arginine methyl ester (L-NAME, 70 mg/kg, i.p., an inhibitor of NO synthase), N-ethylmaleimide NEM, 10 mg/kg, s.c., a blocker of SHs), or indomethacin (10 mg/kg, s.c., an inhibitor of PGs biosynthesis). The attenuation of L-NAME was abolished by L-arginine (300 mg/kg, i.v., a substrate of NO synthase but not by D-arginine (300 mg/kg, i.v., the enatiomer of L-arginine). The effect of the combination of capsaicin with indomethacin, NEM, or L-NAME was not more potent than that of capsaicin alone. The combination of indomethacin and NEM, indomethacin and L-NAME, or indomethacin and NEM and L-NAME increased the attenuation of each alone. These results suggest that CPSN play an important role in the gastroprotection by momordin Ic on ethanol-induced gastric mucosal lesions, and endogenous PGs, NO, and SHs interactively participate, in rats. (C) 1999 Elsevier Science Inc.