Formation and characterization of phosphatidylethanolamine/lysophosphatidylcholine mixed vesicles.
Formation and characterization of phosphatidylethanolamine/lysophosphatidylcholine mixed vesicles.
复制标题
磷脂酰乙醇胺/溶血磷脂酰胆碱混合囊泡的形成和表征。
DOI：
10.1016/s0005-2736(00)00192-9
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
M. Ueno
中科院分区：
文献类型：
--
作者：
C. Sun;A. Hanasaka;H. Kashiwagi;M. Ueno
在磷脂酰乙醇胺(PE)分散体中加入溶血磷脂酰胆碱(LysoPC)溶液，在4℃下加入溶血磷脂酰胆碱(LysoPC)溶液，然后在37℃孵育，从负染的电子显微镜和钙黄绿素的荧光潜伏期判断，所形成的脂质聚集体为囊泡系统。所得结果类似于对磷脂酰胆碱(PC)囊泡的描述。层析结果表明，PE/lysoPC囊泡中PE和lysoPC的掺入摩尔比为5：2。PE/lysoPC膜对氯离子−或钙黄绿素的阻隔电位与PC膜相似。1H核磁共振测量表明，溶质PC占据囊泡的外单分子层。此外，还发现在PE/lysoPC囊泡悬浮液中加入大量的lysoPC可以使PE/lysoPC囊泡和胶束共存。讨论了PE/lysoPC囊泡的形成，并结合PE膜上的lysoPC对层间附着的抑制作用进行了讨论。
The lipid aggregates formed by adding lysophosphatidylcholine (lysoPC) solution to phosphatidylethanolamine (PE) dispersion at 4°C followed by incubating it at 37°C were proved to be a vesicle system judged from the negatively stained electron micrographs and the latency of calcein fluorescence. The results obtained are analogous to those described for phosphatidylcholine (PC) vesicles. The chromatography results showed that the incorporation of PE and lysoPC into the PE/lysoPC vesicles was in a molar ratio of 5 to 2. The PE/lysoPC membrane was found to have similar barrier potentials for Cl−or calcein efflux to the PC membrane.1H Nuclear magnetic resonance measurement suggested that lysoPC dominated the external monolayer of the vesicles. Furthermore, it was found that PE/lysoPC vesicles and micelles could coexist when a large amount of lysoPC was added to the PE/lysoPC vesicle suspension. The formation of PE/lysoPC vesicles is discussed in combination with the inhibition of interlayer attachment by lysoPC from the PE membrane.
脂质成分对肌浆网Ca2-ATP酶钙/腺苷5-三磷酸偶联比的影响。
影响因子：
2.9
作者：
Navarro,J;Toivio-Kinnucan,M;Racker,E
通讯作者：
Racker,E
影响因子：
2.9
作者：
Yeagle,PL;Smith,FT;Young,JE;Flanagan,TD
通讯作者：
Flanagan,TD