Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH.
Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH.
复制标题
脑室内注射TRH后大鼠脑中脱酰胺-TRH（焦谷氨酰-组氨酰-脯氨酸）的形成和降解。
DOI：
10.1016/0006-8993(81)90136-0
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Wurtman,RJ
中科院分区：
文献类型：
--
作者：
Spindel,ER;Lakher,M;Wurtman,RJ
将促甲状腺激素释放激素（TRH）注入大鼠侧脑室，迅速代谢为脱氨基TRH（DA-TRH）。脑水平的TRH下降的半衰期为7分钟，而DA-TRH形成的，它消失了，半衰期为2.5分钟。为了防止死后降解的DA-TRH，有必要牺牲大鼠直接集中微波照射对大脑。TRH和DA-TRH在体内测定的短半衰期比使用体外技术获得的短得多。在体内DA-TRH的形成和积累的抑制杆菌肽和丙磺舒的影响。
Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from it disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.