A respiratory syncytial virus (RSV) vaccine based on parainfluenza virus 5 (PIV5).
A respiratory syncytial virus (RSV) vaccine based on parainfluenza virus 5 (PIV5).
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基于副流感病毒 5 (PIV5) 的呼吸道合胞病毒 (RSV) 疫苗。
DOI：
10.1016/j.vaccine.2014.03.049
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发表时间：
2014
期刊：
影响因子：
5.5
通讯作者：
He,Biao
中科院分区：
文献类型：
--
作者：
Phan,ShannonI;Chen,Zhenhai;Xu,Pei;Li,Zhuo;Gao,Xiudan;Foster,StephanieL;Teng,MichaelN;Tripp,RalphA;Sakamoto,Kaori;He,Biao
人呼吸道合胞病毒（RSV）是婴幼儿严重呼吸道疾病和住院的主要原因。它还在老年人和免疫受损的个体中引起显著的发病率和死亡率。目前还没有获得许可的疫苗。副流感病毒5（PIV 5）是一种副粘病毒，不会引起已知的人类疾病，并已被用作基于载体的疫苗开发的平台。为了评价PIV 5作为RSV疫苗载体的功效，我们产生了两种重组PIV 5病毒-一种表达融合（F）蛋白，另一种表达RSV毒株A2（RSV A2）的附着糖蛋白（G）。疫苗株分别用于BALB/c小鼠的单次接种。结果显示，两种疫苗均诱导RSV抗原特异性抗体应答，IgG 2a/IgG 1比值与野生型RSV A2感染中观察到的相似。在用RSV A2攻击接种疫苗的小鼠后，肺切片的组织病理学显示，相对于RSV A2免疫的小鼠，疫苗没有加重肺病变。重要的是，F和G疫苗均诱导保护性免疫。因此，PIV 5为针对RSV感染的基于载体的疫苗提供了一个有吸引力的平台。
Human respiratory syncytial virus (RSV) is a leading cause of severe respiratory disease and hospitalizations in infants and young children. It also causes significant morbidity and mortality in elderly and immune compromised individuals. No licensed vaccine currently exists. Parainfluenza virus 5 (PIV5) is a paramyxovirus that causes no known human illness and has been used as a platform for vector-based vaccine development. To evaluate the efficacy of PIV5 as a RSV vaccine vector, we generated two recombinant PIV5 viruses – one expressing the fusion (F) protein and the other expressing the attachment glycoprotein (G) of RSV strain A2 (RSV A2). The vaccine strains were used separately for single-dose vaccinations in BALB/c mice. The results showed that both vaccines induced RSV antigen-specific antibody responses, with IgG2a/IgG1 ratios similar to those seen in wild-type RSV A2 infection. After challenging the vaccinated mice with RSV A2, histopathology of lung sections showed that the vaccines did not exacerbate lung lesions relative to RSV A2-immunized mice. Importantly, both F and G vaccines induced protective immunity. Therefore, PIV5 presents an attractive platform for vector-based vaccines against RSV infection.