alpha-Amanitin inhibits the oxidation of long chain fatty acids in mouse liver.
alpha-Amanitin inhibits the oxidation of long chain fatty acids in mouse liver.
复制标题
α-鹅膏蕈碱抑制小鼠肝脏中长链脂肪酸的氧化。
DOI：
10.1093/oxfordjournals.jbchem.a133488
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发表时间：
1981
影响因子：
2.7
通讯作者：
S. Furuta
中科院分区：
文献类型：
--
作者：
T. Hashimoto;S. Miyazawa;D. Gunarso;S. Furuta
结果发现，给小鼠注射毒鹅膏菌提取物可抑制肝线粒体对长链脂肪酸的氧化。抑制原理被鉴定为α-鹅膏蕈碱。在注射相当于LD 50的约0.2 mg/kg水平的α-鹅膏蕈碱后数小时观察到抑制。肉毒碱酰基转移酶、酰基-CoA脱氢酶、烯酰-CoA水合酶、3-羟酰基-CoA脱氢酶和3-酮酰基-CoA硫解酶的活性保持恒定。使用具有不同碳链长度的脂肪酸作为底物，长链脂肪酸的氧化被显著抑制，伴随着肉毒碱依赖性组分的消失。中链和短链脂肪酸的氧化减少不太明显。因此，它建议，该网站的抑制是长链酰基肉毒碱的运输。过氧化物酶体β-氧化不受α-鹅膏蕈碱处理的影响。
It was found that the injection of the extract of Amanita virosa mushroom into mice resulted in the inhibition of long chain fatty acid oxidation by liver mitochondria. The inhibition principle was identified as alpha-amanitin. The inhibition was observed several hours after the injection of alpha-amanitin at the level of about 0.2 mg/kg, which corresponded to LD50. Activities of carnitine acyltransferases, acyl-CoA dehydrogenases, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase remained constant. Using fatty acids with various carbon chain lengths as substrates, the oxidation of long chain fatty acids was markedly inhibited with accompanying disappearance of the carnitine-dependent component. The decreases in the oxidation of medium and short chain fatty acids were less marked. It is suggested, therefore, that the site of the inhibition is the transport of long chain acylcarnitines. Peroxisomal beta-oxidation was not affected by the alpha-amanitin treatment.