Genetic difference in susceptibility to the blood-retina barrier breakdown in diabetes and oxygen-induced retinopathy
Genetic difference in susceptibility to the blood-retina barrier breakdown in diabetes and oxygen-induced retinopathy
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DOI：
10.1016/s0002-9440(10)62255-9
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发表时间：
2005-01-01
影响因子：
6
通讯作者：
Lambrou, GN
中科院分区：
文献类型：
--
作者：
Zhang, SX;Ma, JX;Lambrou, GN
血-视网膜屏障（BRB）的破坏是糖尿病视网膜病变的常见特征。本研究的目的是确定是否有遗传差异的易感性的BRB在糖尿病视网膜病变的故障使用两种大鼠模型。在链脲佐菌素（STZ）诱导的糖尿病中，布朗挪威（BN）大鼠在整个实验期间（糖尿病的16周）在视网膜中发展出持续的血管通透性过高，而糖尿病Sprague道利（SD）大鼠仅在糖尿病发作后3 - 10天显示视网膜通透性过高。在这两个菌株中，渗透性的菌株差异与血糖水平无关。在氧诱导的视网膜病变（OIR）中，BN大鼠从出生后12天（P12）到P22发生视网膜血管通透性增高，在P16达到峰值，是年龄匹配的正常对照组的8.7倍。然而，在OIR-SD大鼠中，从P14至P18观察到高通透性，峰值仅为对照组的2.2倍。两种模型视网膜血管通透性的差异与血管内皮生长因子（VEGF）的不同表达有关。这一发现表明，遗传背景有助于糖尿病视网膜病变的易感性。
The breakdown of the blood-retina barrier (BRB) is a common feature of diabetic retinopathy. The purpose of the present study is to determine whether there are genetic differences in susceptibility to the breakdown of the BRB in diabetic retinopathy using two rat models. in streptozotocin (STZ)-induced diabetes, Brown Norway (BN) rats developed sustained vascular hyperpermeability in the retina during the entire experimental period (16 weeks of diabetes), while diabetic Sprague Dawley (SD) rats only showed retinal hyperpermeability from 3 to 10 days after the onset of diabetes. The strain difference in permeability was not correlated with the blood glucose levels in these two strains. In oxygen-induced retinopathy (OIR), BN rats developed retinal vascular hyperpermeability from postnatal day 12 (P12) to P22 with a peak at P16, which was 8.7-fold higher than that in the age-matched normal controls. In OIR-SD rats, however, hyperpermeability was observed from P14 to P18, with a peak only 2.2-fold higher than that in the controls. The strain difference in vascular hyperpermeability was correlated with the different overexpression of vascular endothelial growth factor (VEGF) in the retina of these two models. This finding suggests that genetic backgrounds contribute to the susceptibility to diabetic retinopathy.