The pharmacology of efferent synapses in the lateral-line system of Xenopus laevis.
The pharmacology of efferent synapses in the lateral-line system of Xenopus laevis.
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非洲爪蟾侧线系统传出突触的药理学。
DOI：
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发表时间：
1971
影响因子：
2.8
通讯作者：
I. Russell
中科院分区：
文献类型：
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作者：
I. Russell
1.在侧线传出突触释放的递质物质的性质进行了研究，在非洲爪蟾和Acerina的组织化学技术和非洲爪蟾的药理学方法。
2.基地的侧线毛细胞和细纤维的侧线神经的乙酰胆碱酯酶的反应呈阳性Acerina和在较小程度上在爪蟾。
3.乙酰胆碱（10- 6 M）和乙酰-β-甲基胆碱（5 × 10- 6 M）具有毒蕈碱作用，当移液到侧线缝线的下侧时，对传入冲动产生强烈的可逆性抑制。卡巴胆碱（5 × 10-4至5 × 10- 5 M）引起的自发传入冲动的可逆性抑制较小，但其他烟碱类物质（丙酰胆碱和丁酰胆碱）没有影响。
4.毒扁豆碱（5 × 10- 5 M）延长了电刺激传出纤维对传入冲动的抑制，但阿托品（5 × 10- 6 M）阻断了这种抑制。
5.钙和镁在传出突触中的相互作用与在两栖动物神经肌肉接头中发现的方式相似。
6.提出了论据来支持乙酰胆碱在侧线传出突触释放的假设。
1. The nature of the transmitter substance released at the lateral-line efferent synapses was investigated by histochemical techniques in Xenopus and Acerina and by pharmacological methods in Xenopus .
2. The bases of lateral-line hair-cells and fine fibres in the lateral-line nerves reacted positively for acetylcholinesterase in Acerina and to a lesser extent in Xenopus .
3. Acetylcholine (10-6M, and acetyl-β-methyl choline (5 x 10-6M), which has a muscarinic action, caused strong reversible inhibition of afferent impulses when pipetted on to the undersides of lateral-line stitches. Carbachol (5 x 10-4 to 5 x 10-5M) caused a smaller reversible inhibition of spontaneous afferent impulses, but other nicotinic substances (propionylcholine and buterylcholine) had no effect.
4. Physostigmine (5 x 10-5M) prolonged inhibition of afferent impulses by electrical stimulation of efferent fibres, but atropine (5 x 10-6M) blocked it.
5. Calcium and magnesium interact at the efferent synapses in a way similar to that found at the amphibian neuromuscular junction.
6. Arguments are put forward to support the hypothesis that acetylcholine is released at lateral-line efferent synapses.