Reelin-expressing neurons in the anterior commissure and corpus callosum of the rat
Reelin-expressing neurons in the anterior commissure and corpus callosum of the rat
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DOI：
10.1016/j.devbrainres.2003.10.009
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发表时间：
2004-01-31
期刊：
影响因子：
--
通讯作者：
Terashima, T
中科院分区：
文献类型：
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作者：
Misaki, K;Kikkawa, S;Terashima, T
Reelin是一种细胞外基质蛋白，在中枢神经系统层状和非层状结构的形成中起着至关重要的作用。为了阐明Reelin在出生后大脑中的作用，本研究制备了Reelin的多克隆抗体，并对出生后大鼠大脑中Reelin表达神经元进行了详细的研究。结果发现，在胼胝体和前连合中有表达Reelin的细胞。这些表达Reelin的细胞也用对神经元特异的抗体进行免疫染色，但用对星形胶质细胞和少突胶质细胞特异的抗体不进行免疫染色，表明这些在白色物质中表达Reelin的细胞是神经元。它们也用抗GAD 67抗体免疫染色，表明连合系统中表达Reelin的细胞是GABA能神经元。前连合中的Reelin表达神经元在其树突上有许多明显的静脉曲张，与束间神经元相似。这些结果表明，生后Reelin可能通过连合结构参与神经元活动的调节机制。(C)2003 Elsevier B.V.保留所有权利。
Reelin is an extracellular matrix protein, which plays a crucial role for the formation of laminated and nonlaminated structures in the central nervous system. To elucidate its roles in the postnatal brain, in the present study, we raised a polyclonal antibody specific for rat Reelin, and investigated Reelin-expressing neurons in the rat brain during the postnatal periods in detail. We found that some Reelin-expressing cells existed in the anterior commissure and corpus callosum. These Reelin-expressing cells were also immunostained with the antibody specific for neurons, but not immunostained with the antibodies specific for astrocytes nor oligodendrocytes, suggesting that these Reelin-expressing cells in the white matter are neurons. They are also immunostained with anti-GAD67 antibody, indicating that Reelin-expressing cells in the commissure systems are GABAergic neurons. Reelin-expressing neurons in the anterior commissure had many conspicuous varicosities on their dendritic arbors and mimic to the interfascicular neurons. These results suggest that Reelin may participate in the regulatory mechanism of neuronal activities through the commissure structure during the postnatal periods. (C) 2003 Elsevier B.V. All rights reserved.