Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine
Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine
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DOI：
10.1016/j.devcel.2004.05.007
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发表时间：
2004-06-01
影响因子：
11.8
通讯作者：
McClatchey, AI
中科院分区：
文献类型：
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作者：
Saotome, I;Curto, M;McClatchey, AI
Ezrin、Radixin和Moesin（ERM蛋白）提供膜蛋白和肌动蛋白细胞骨架之间的调节连接。哺乳动物ERM蛋白的研究一直受到假定的功能重叠的阻碍。我们已经发现Ezrin是在发育中的肠的上皮细胞中检测到的唯一的ERM，其在小鼠肠上皮的构造中起重要作用。令人惊讶的是，Ezrin不是刷状缘微绒毛形成或上皮极性的建立或维持所绝对需要的。相反，Ezrin组织顶端末端网区域，这对于绒毛形态发生期间从头管腔形成和扩张的过程知之甚少是至关重要的。我们的数据还表明，埃兹蛋白控制的定位和/或功能的某些顶端膜蛋白，支持正常的肠道功能。这些体内研究强调了Ezrin在多细胞上皮而不是单个上皮细胞形成中的关键功能。
Ezrin, Radixin, and Moesin (the ERM proteins) supply regulated linkage between membrane proteins and the actin cytoskeleton. The study of mammalian ERM proteins has been hampered by presumed functional overlap. We have found that Ezrin, the only ERM detected in epithelial cells of the developing intestine provides an essential role in configuring the mouse intestinal epithelium. Surprisingly, Ezrin is not absolutely required for the formation of brush border microvilli or for the establishment or maintenance of epithelial polarity. Instead, Ezrin organizes the apical terminal web region, which is critical for the poorly understood process of de novo lumen formation and expansion during villus morphogenesis. Our data also suggest that Ezrin controls the localization and/or function of certain apical membrane proteins that support normal intestinal function. These in vivo studies highlight the critical function of Ezrin in the formation of a multicellular epithelium rather than an individual epithelial cell.