Expression of estrogen receptors in gastric cancer and their clinical significance
Expression of estrogen receptors in gastric cancer and their clinical significance
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DOI：
10.1002/jso.23097
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发表时间：
2012-09-01
影响因子：
2.5
通讯作者：
Kim, Chong-Suk
中科院分区：
文献类型：
--
作者：
Ryu, Woo-Sang;Kim, Jong-Han;Kim, Chong-Suk
背景与目的胃癌的男性优势提示女性性激素可能对胃癌有保护作用。本研究旨在探讨雌激素受体在胃癌组织和细胞中的表达及其临床意义。方法采用蛋白质印迹法分析从正常胃、胃癌组织和培养的胃癌细胞（KATO-III、mkn 28、mkn 45和mkn 74）中提取的ER-α、ER-β蛋白。应用组织芯片技术和免疫组化染色方法对148例胃癌组织中ER-β的表达进行了研究。结果ER-α和ER-β蛋白在正常组织和胃癌组织中均有表达。然而，在培养的胃细胞中，仅在mkn 28和mkn 74中观察到ER-β。148例胃癌中ER β阳性67例（45.3%）。ER-β阳性组与肿瘤分期较低、Lauren肠型、神经浸润阴性和无复发相关。生存分析显示ER-β阳性组3年生存率高于阴性组。结论胃癌组织中ER-β的存在可能对胃癌的侵袭性具有保护作用。需要进一步的研究来阐明ER-β在胃癌中的作用。外科肿瘤学杂志2012; 106：456461. (c)2012 Wiley Periodicals，Inc.
Backgrounds and Objectives The male predominance of gastric cancer suggests that female sex hormones may have a protective effect against gastric cancer. We evaluated the expression of estrogen receptors in gastric cancer tissue and cells and the clinical significance of ER-beta expression in gastric cancer. Method ER-alpha, ER-beta proteins extracted from normal stomach, gastric cancer tissues, and cultured gastric cancer cells (KATO-III, mkn28, mkn45, and mkn74) were assessed by Western blot analysis. The clinical significance of ER-beta was explored using tissue microarray methods and immunohistochemical staining of specimens from 148 gastric cancers. Results Both ER-alpha and beta protein expression were noted in normal and gastric cancer tissues. However, in cultured gastric cells, only ER-beta was noted in mkn28 and mkn74. Of 148 gastric cancers, 67 (45.3%) were ER-beta positive. The ER-beta positive group was associated with lower tumor stage, Lauren's intestinal type, negative perineural invasion, and free of recurrence. The ER-beta positive group had a better 3-year survival compared with the negative group in survival analysis. Conclusion Our results suggest that the presence of ER-beta in gastric cancer could have a protective effect against invasiveness of gastric cancer. Further studies are needed to clarify the role of ER-beta in gastric cancers. J. Surg. Oncol. 2012; 106:456461. (c) 2012 Wiley Periodicals, Inc.