Dietary protein restriction regulates skeletal muscle fiber metabolic characteristics associated with the FGF21-ERK1/2 pathway
Dietary protein restriction regulates skeletal muscle fiber metabolic characteristics associated with the FGF21-ERK1/2 pathway
复制标题
饮食蛋白质限制调节与 FGF21-ERK1/2 通路相关的骨骼肌纤维代谢特征
DOI：
10.1016/j.isci.2024.109249
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发表时间：
2024-03-02
期刊：
影响因子：
5.8
通讯作者：
You，Jinming
中科院分区：
文献类型：
--
作者：
Li，Shuo;Zhong，Haopeng;You，Jinming
在日粮氨基酸平衡的条件下，降低日粮粗蛋白水平有利于改善肉质。为了进一步阐明这一机制，我们探索了饲喂蛋白质限制日粮的猪骨骼肌中肌纤维特性和与肌肉发生相关的关键调控因子的变化。与正常蛋白质日粮相比，蛋白质限制显著提高了猪骨骼肌中慢收缩肌纤维比例、琥珀酸脱氢酶活性、抗坏血酸、生物素、棕榈油酸浓度和S-同型半胱氨酸/S-同型半胱氨酸比值，但降低了快收缩肌纤维比例、乳酸脱氢酶活性、骨骼肌中三磷酸腺苷、葡萄糖-6-磷酸、琥珀酸脱氢酶和琥珀酸脱氢酶浓度，降低了血清三碘甲状腺原氨酸(T3)/四碘甲状腺原氨酸(T4)的比值。综上所述，我们证明了饲料蛋白限制诱导的断奶仔猪骨骼肌纤维重构与FGF21-ERK1/2-mTORC1信号的调节有关。
Under conditions of dietary amino acid balance, decreasing the dietary crude protein (CP) level in pigs has a beneficial effect on meat quality. To further elucidate the mechanism, we explored the alteration of muscle fiber characteristics and key regulators related to myogenesis in the skeletal muscle of pigs fed a protein restricted diet. Compared to pigs fed a normal protein diet, dietary protein restriction significantly increased the slow-twitch muscle fiber proportion in skeletal muscle, succinic dehydrogenase (SDH) activity, the concentrations of ascorbate, biotin, palmitoleic acid, and the ratio of s-adenosylhomocysteine (SAM) to s-adenosylhomocysteine (SAH), but the fast-twitch muscle fiber proportion, lactate dehydrogenase (LDH) activity, the concentrations of ATP, glucose-6-phosphate, SAM, and SAH in skeletal muscle, and the ratio of serum triiodothyronine (T3) to tetraiodothyronine (T4) were decreased. In conclusion, we demonstrated that dietary protein restriction induced skeletal muscle fiber remodeling association the regulation of FGF21-ERK1/2-mTORC1 signaling in weaned piglets.