Adrenal cortex transplantation after bilateral total adrenalectomy in the rat.
Adrenal cortex transplantation after bilateral total adrenalectomy in the rat.
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大鼠双侧肾上腺全切除术后肾上腺皮质移植。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
Soren Schroder
中科院分区：
文献类型：
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作者：
Georg F. W. Scheumann;Wolfgang F. A. Hiller;Soren Schroeder;T. Schurmeyer;Jurgen Klempnauer;H. Dralle;Georg F. W. Scheumann;Wolfgang F. A. Hiller;Soren Schroder
建立了肾上腺皮质移植的实验动物模型，以研究肾上腺皮质替代疗法对因嗜铬细胞瘤而接受双侧肾上腺切除术的多发性内分泌瘤2型患者的治疗作用。通过胶原酶消化和确定的沉降，从髓质分离同源大鼠的肾上腺皮质。将皮质细胞的细胞悬液植入未处理的同系大鼠的肾囊下。两周后，受体双侧肾上腺切除。血清皮质酮水平作为移植物功能的估计。在整个观察期间，所有受体都是健康的，而所有肾上腺切除的对照组在18天内死亡。免疫组化显示移植物中有存活的皮质细胞。与对照组（0.20 ng/mL +/- 0.06）相比，移植动物的皮质酮水平接近正常（9.5 ng/100 mL +/-0.4）。这种肾上腺皮质移植的动物模型允许从皮质细胞分离髓质细胞。移植后，这些皮质细胞存活了8周，并能够取代肾上腺皮质功能。
An experimental animal model with adrenal cortex transplantation was developed to study adrenal cortex replacement therapy in patients with multiple endocrine neoplasia type 2 who have had bilateral adrenalectomy for pheochromocytomas. Adrenal cortex of syngenetic rats was isolated from the medulla by collagenase digestion and a defined sedimentation. The cell suspension of the cortical cells was implanted under the kidney capsule of untreated syngenetic rats. After two weeks the recipients were bilaterally adrenalectomized. Serum corticosterone levels were measured as an estimate of function of the grafts. All recipients were healthy throughout the observation period, whereas all adrenalectomized controls died within 18 days. Vital cortex cells could be demonstrated in the explanted grafts by immunohistochemistry. Corticosterone levels of transplanted animals were nearly normal (9.5 ng/100 mL +/- 0.4) compared to the controls (0.20 ng/mL +/- 0.06). This animal model of adrenal cortex transplantation allows the separation of medullary from cortical cells. After transplantation, these cortical cells survived for eight weeks and were able to replace the adrenal cortex function.