The responses of extrinsic fibroblasts infiltrating the devitalised patellar tendon to IL-1beta are different from those of normal tendon fibroblasts.
The responses of extrinsic fibroblasts infiltrating the devitalised patellar tendon to IL-1beta are different from those of normal tendon fibroblasts.
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DOI：
10.1302/0301-620x.89b9.18053
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
H. Tohyama;K. Yasuda;H. Uchida;J. Nishihira†
中科院分区：
文献类型：
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作者：
H. Tohyama;K. Yasuda;H. Uchida;J. Nishihira†
为了阐明细胞因子在韧带重建移植肌腱重构中的作用，我们采用Northern blot分析方法，比较了大鼠冻融髌骨肌腱外源性成纤维细胞对白细胞介素(IL)-1 β、血小板衍生生长因子(PDGF)-BB和转化生长因子(TGF)- β 1的反应，以及基质金属蛋白酶(MMP)-13基因的表达。我们还通过免疫组织学检查了il -1 β、PDGF-BB和tgf - β 1在浸润冻融髌骨肌腱的成纤维细胞中的局部表达。Northern blot分析显示，原位冻融后6周的髌腱成纤维细胞在mmp - 13mrna基因表达方面对il -1 β的反应低于正常肌腱成纤维细胞。免疫组织学结果显示，在原位冻融后2周和6周浸润髌骨肌腱的外源性成纤维细胞中il -1 β过表达，但PDGF-BB和tgf - β 1在这些外源性成纤维细胞中均未过表达。我们的研究结果表明，除了组织愈合过程中炎症的开始外，il -1 β与用于韧带重建的移植肌腱的基质重塑密切相关。
In order to clarify the role of cytokines in the remodelling of the grafted tendon for ligament reconstruction we compared the responses to interleukin (IL)-1beta, platelet-derived growth factor (PDGF)-BB and transforming growth factor (TGF)-beta1 of extrinsic fibroblasts infiltrating the frozen-thawed patellar tendon in rats with that of the normal tendon fibroblasts, in regard to the gene expression of matrix metalloproteinase (MMP)-13, using Northern blot analysis. We also examined, immunohistologically, the local expression of IL-1beta, PDGF-BB, and TGF-beta1 in fibroblasts infiltrating the frozen-thawed patellar tendon. Northern blot analysis showed that fibroblasts derived from the patellar tendon six weeks after the freeze-thaw procedure in situ showed less response to IL-1beta than normal tendon fibroblasts with respect to MMP-13 mRNA gene expression. The immunohistological findings revealed that IL-1beta was over-expressed in extrinsic fibroblasts which infiltrated the patellar tendon two and six weeks after the freeze-thaw procedure in situ, but neither PDGF-BB nor TGF-beta1 was over-expressed in these extrinsic fibroblasts. Our findings indicated that IL-1beta had a close relationship to matrix remodelling of the grafted tendon for ligament reconstruction, in addition to the commencement of inflammation during the tissue-healing process.