Preparation of testate amoebae samples affects water table depth reconstructions in peatland palaeoecological studies
Preparation of testate amoebae samples affects water table depth reconstructions in peatland palaeoecological studies
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DOI：
10.3176/earth.2013.09
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发表时间：
2013
影响因子：
1.1
通讯作者：
Eve Avel;M. Pensa
中科院分区：
文献类型：
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作者：
Eve Avel;M. Pensa
在泥炭地古生态学研究中，用于有壳变形虫（TA）分析的泥炭样品的制备包括将样品煮沸并通过15微米的筛子进行微筛。我们研究了这些准备阶段对TA组合的估计和地下水位深度（WTD）的重建的影响。我们的研究结果表明，煮沸和未煮沸的样品的TA组合没有显着不同，而微筛降低了小TA类群的浓度，并导致显着不同的TA组合。微筛和未筛分的TA组合之间的差异也反映在预测值的WTD，这表明干燥条件的情况下，未筛分的样品比在微筛样品。我们的结论是，样品的煮沸可以省略，如果TA是从新鲜泥炭样品中提取。微筛可能会导致错误的古生态WTD重建，应避免，如果小TA类群存在于样品中。
In peatland palaeoecological studies, the preparation of peat samples for testate amoebae (TA) analysis involves boiling of samples and microsieving them through a 15-µm sieve. We studied the effect of these preparation stages on the estimation of TA assemblages and on the reconstruction of water table depths (WTD). Our results indicate that the TA assemblages of boiled and unboiled samples are not significantly different, while microsieving reduces the concentration of small TA taxa and results in significantly different TA assemblages. The differences between microsieved and unsieved TA assemblages were reflected also in predicted values of WTD, which indicated drier conditions in case of unsieved samples than in microsieved samples. We conclude that the boiling of samples might be omitted if TA are extracted from the fresh peat samples. Microsieving may lead to erroneous palaeoecological WTD reconstructions and should be avoided if small TA taxa are present in samples.