Genome-wide analysis of maize GPAT gene family and cytological characterization and breeding application of ZmMs33/ZmGPAT6 gene
Genome-wide analysis of maize GPAT gene family and cytological characterization and breeding application of ZmMs33/ZmGPAT6 gene
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玉米GPAT基因家族全基因组分析及ZmMs33/ZmGPAT6基因的细胞学表征及育种应用
DOI：
10.1007/s00122-019-03343-y
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发表时间：
2019
影响因子：
5.4
通讯作者：
Wan Xiangyuan
中科院分区：
文献类型：
--
作者：
Zhu Taotao;Wu Suowei;Zhang Danfeng;Li Ziwen;Xie Ke;An Xueli;Ma Biao;Hou Quancan;Dong Zhenying;Tian Youhui;Li Jinping;Wan Xiangyuan
玉米GPAT基因家族的全基因组分析、编码er定位蛋白的ZmMs33/ZmGPAT6基因的细胞学特征及其在玉米中的分子育种应用甘油-3-磷酸酰基转移酶（Glycerol-3-phosphate acyltransferase， GPAT）介导了甘油脂生物合成的初始步骤，在植物生长发育中起着关键作用。与拟南芥GPAT基因相比，我们对玉米GPAT基因家族的了解非常有限。最近，我们的实验室已经鉴定出ZmMs33基因编码sn-2 GPAT蛋白并控制玉米雄性育性（Xie et al. in theappl Genet 131:1363-1378, 2018）。然而，ZmMs33的作用机制尚不清楚。本文报道了玉米GPAT基因家族的全基因组分析，发现20个玉米GPAT基因（ZmGPAT1-20）可以被划分为3个不同的分支，与拟南芥中的10个GPAT基因相似。ZmGPAT基因在6个组织和花药中6个发育阶段的表达分析表明，部分ZmGPAT基因可能在玉米生长和花药发育中起关键作用。其中ZmGPAT6对应ZmMs33基因。系统细胞学观察表明，ZmMs33/ZmGPAT6基因功能缺失导致玉米花药角质层缺陷，花药壁层退化受阻，乌氏体和外壁形成异常，最终导致玉米雄性不育。内质网定位的ZmMs33/ZmGPAT6具有4个保守的酰基转移酶活性必需氨基酸基序，而ZmMs33/ZmGPAT6位点及其周围的基因组区域在草属物种的进化过程中发生了极大的多样化。最后，利用转基因和标记辅助选择相结合的策略，建立了ms33雄性不育系的多控制系统。该研究将为进一步解读ZmGPAT基因的功能机制和ZmMs33/ZmGPAT6基因在玉米中的分子育种应用提供有益信息。
Genome-wide analysis of maize GPAT gene family, cytological characterization of ZmMs33/ZmGPAT6 gene encoding an ER-localized protein with four conserved motifs, and its molecular breeding application in maize. Glycerol-3-phosphate acyltransferase (GPAT) mediates the initial step of glycerolipid biosynthesis and plays pivotal roles in plant growth and development. Compared with GPAT genes in Arabidopsis, our understanding to maize GPAT gene family is very limited. Recently, ZmMs33 gene has been identified to encode a sn-2 GPAT protein and control maize male fertility in our laboratory (Xie et al. in Theor Appl Genet 131:1363-1378, 2018). However, the functional mechanism of ZmMs33 remains elusive. Here, we reported the genome-wide analysis of maize GPAT gene family and found that 20 maize GPAT genes (ZmGPAT1-20) could be classified into three distinct clades similar to those of ten GPAT genes in Arabidopsis. Expression analyses of these ZmGPAT genes in six tissues and in anther during six developmental stages suggested that some of ZmGPATs may play crucial roles in maize growth and anther development. Among them, ZmGPAT6 corresponds to the ZmMs33 gene. Systemic cytological observations indicated that loss function of ZmMs33/ZmGPAT6 led to defective anther cuticle, arrested degeneration of anther wall layers, abnormal formation of Ubisch bodies and exine and ultimately complete male sterility in maize. The endoplasmic reticulum-localized ZmMs33/ZmGPAT6 possessed four conserved amino acid motifs essential for acyltransferase activity, while ZmMs33/ZmGPAT6 locus and its surrounding genomic region have greatly diversified during evolution of gramineous species. Finally, a multi-control sterility system was developed to produce ms33 male-sterile lines by using a combination strategy of transgene and marker-assisted selection. This work will provide useful information for further deciphering functional mechanism of ZmGPAT genes and facilitate molecular breeding application of ZmMs33/ZmGPAT6 gene in maize.