Dietary fish oil alters cardiomyocyte Ca2+ dynamics and antioxidant status
Dietary fish oil alters cardiomyocyte Ca2+ dynamics and antioxidant status
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DOI：
10.1016/j.freeradbiomed.2005.12.026
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发表时间：
2006-05-01
影响因子：
7.4
通讯作者：
McMurchie, EJ
中科院分区：
文献类型：
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作者：
Jahangiri, A;Leifert, WR;McMurchie, EJ
鱼油 (FO) 中发现的 n-3 多不饱和脂肪酸 (PUFA) 已被证明可以预防再灌注心律失常，这是再灌注损伤的一种表现，据信这是由活性氧 (ROS) 的形成和细胞内钙 (Ca2+) 过载引起的。与补充饱和脂肪 (SF) 的组相比，饲喂补充 10% FO 的饮食的成年大鼠心肌 n-3 PUFA 比例更高，抗氧化酶表达增加。与FO组相比，向SF补充组大鼠分离的心肌细胞中添加过氧化氢（H2O2）可增加心肌细胞异步收缩的比例，增加Ca2+流入速率，并增加舒张压和收缩压[Ca2+](i)。 H2O2 暴露增加了 FO 组心肌细胞的膜流动性。这些结果表明，饮食中补充 FO 与降低心肌细胞对 ROS 诱导损伤的敏感性有关，这可能与 n-3 PUFA 的膜掺入、抗氧化防御能力增强、心肌细胞膜流动性变化以及防止细胞 Ca2+ 响应 ROS 升高的能力有关。 (c) 2006 Elsevier Inc. 保留所有权利。
The n-3 polyunsaturated fatty acids (PUFAs) found in fish oil (FO) have been shown to protect against reperfusion arrhythmias, a manifestation of reperfusion injury, which is believed to be induced by the formation of reactive oxygen species (ROS) and intracellular calcium (Ca2+) overload. Adult rats fed a diet supplemented with 10% FO had a higher proportion of myocardial n-3 PUFAs and increased expression of antioxidant enzymes compared with the saturated fat (SF)-supplemented group. Addition of hydrogen peroxide (H2O2) to cardiomyocytes isolated from rats in the SF-supplemented group increased the proportions of cardiomyocytes contracting in an asynchronous manner, increased the rate of Ca2+ influx, and increased the diastolic and systolic [Ca2+](i) compared with the FO group. H2O2 exposure increased the membrane fluidity of cardiomyocytes from the FO group. These results demonstrate that dietary FO supplementation is associated with a reduction in the susceptibility of myocytes to ROS-induced injury and this may be related to membrane incorporation of n-3 PUFAs, increased antioxidant defenses, changes in cardiomyocyte membrane fluidity, and the ability to prevent rises in cellular Ca2+ in response to ROS. (c) 2006 Elsevier Inc. All rights reserved.