A novel transcription factor involved in plant defense endowed with protein phosphatase activity
A novel transcription factor involved in plant defense endowed with protein phosphatase activity
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DOI：
10.1093/emboj/cdg323
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发表时间：
2003-07-01
期刊：
影响因子：
11.4
通讯作者：
Vera, P
中科院分区：
文献类型：
--
作者：
Carrasco, JL;Ancillo, G;Vera, P
在植物中，感知病原体后抗病特性的表达涉及大量转录依赖防御的部署。因此，如果要实现快速有效的防御反应，病原信号通过预先存在的信号通路进行转导和放大是至关重要的。在蛋白激酶和磷酸酶的协同作用下，特定转录因子的可逆磷酸化可能是一种快速灵活地调节环境刺激下选择性基因表达的机制。在此，我们从烟草植物中鉴定出一种新的dna结合蛋白，命名为DBP1，具有蛋白磷酸酶活性，它以序列特异性的方式结合到防御相关基因的顺式作用元件并参与其转录调控。这一发现有助于描述植物早期转录依赖防御反应选择性激活的信号通路中的终端事件，并表明调控蛋白的刺激依赖可逆磷酸化可能直接发生在转录蛋白- dna复合物中。
In plants, expression of a disease-resistance character following perception of a pathogen involves massive deployment of transcription-dependent defenses. Thus, if rapid and effective defense responses have to be achieved, it is crucial that the pathogenic signal is transduced and amplified through pre-existing signaling pathways. Reversible phosphorylation of specific transcription factors, by a concerted action of protein kinases and phosphatases, may represent a mechanism for rapid and flexible regulation of selective gene expression by environmental stimuli. Here we identified a novel DNA-binding protein from tobacco plants, designated DBP1, with protein phosphatase activity, which binds in a sequence-specific manner to a cis- acting element of a defense-related gene and participates in its transcriptional regulation. This finding helps delineate a terminal event in a signaling pathway for the selective activation of early transcription-dependent defense responses in plants, and suggests that stimulus-dependent reversible phosphorylation of regulatory proteins may occur directly in a transcription protein-DNA complex.