Trophoblastic giant cells in placentas of rats and mice and their probable role in steroid-hormone production.
Trophoblastic giant cells in placentas of rats and mice and their probable role in steroid-hormone production.
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大鼠和小鼠胎盘中的滋养层巨细胞及其在类固醇激素产生中的可能作用。
作者：
H. W. Deane;B. L. Rubin;E. C. Driks;Bertha L. Lobel;G. Leipsner
采用组织化学和生物化学方法测定了大鼠（妊娠10 - 21天）和小鼠着床部位的Δ 5 - 3 β-羟基类固醇脱氢酶活性。组织化学上，活性仅限于胎儿胎盘的外周巨滋养层细胞。在大鼠中，两种方法均发现酶活性在妊娠约13至15天时达到峰值，此后下降。该酶是C19和C21 Δ 5 - 3 β-羟基类固醇氧化为Δ 4 - 3-酮类固醇所必需的，因此在活性类固醇激素的生物合成中起关键作用。巨细胞的其他特征表明它们在产生类固醇激素中的功能是显著量的细胞质脂质和作为葡萄糖-6-磷酸氧化的结果的高的制造还原的三磷酸吡啶核苷酸（TPNH）的能力。巨细胞的局部侵蚀活动和营养激素的产生也有一些确证性的证据。大鼠巨细胞的特性及其与肝纤维化的关系
Implantation sites of rats (pregnant 10 to 21 days) and of mice have been assayed for Δ5-3β-hydroxysteroid dehydrogenase activity by histochemical and biochemical methods. Histochemically, activity was limited to the peripheral giant trophoblasts of the fetal placenta. In rats, enzymic activity was found by both methods to rise to a peak at about 13 to 15 days of gestation and thereafter to decline. This enzyme is required for oxidation of C19 and C21 Δ5-3β-hydroxysteroids to Δ4-3-ketosteroids and thus plays a key role in the biosynthesis of the active steroid hormones. Other features of the giant cells indicating their function in the production of steroid hormones are a conspicuous amount of cytoplasmic lipid and a high capacity for making reduced triphosphopyridine nucleotide (TPNH) as a result of oxidation of glucose-6-phosphate. Some confirmatory evidence has also been presented for local erosive activity and trophic-hormone production by the giant cells. The attributes of the giant cells in rat and ...