Microfluidic sorting in an optical lattice
Microfluidic sorting in an optical lattice
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DOI：
10.1038/nature02144
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发表时间：
2003-11-27
期刊：
影响因子：
64.8
通讯作者：
Dholakia, K
中科院分区：
文献类型：
--
作者：
MacDonald, MP;Spalding, GC;Dholakia, K
微观介电物体对所施加光场的响应可以深刻地影响其动力学运动（1）。一个经典的例子是光学陷阱，它可以将粒子保持在紧密聚焦的光束中（2）。光场还可以用于在适当的光场几何形状中排列、引导或偏转粒子（3，4）。在这里，我们展示了一个光学分选机的微观粒子，利用粒子的相互作用，生物或其他扩展的，互连的，动态可重构的，三维光晶格。这种与晶格位置的相互作用的强度取决于粒子的光学极化率，从而给出可调的选择标准。我们展示了通过大小（蛋白质微胶囊药物递送剂）和通过折射率（其他胶体颗粒流）进行分选。该方法的分选效率接近100%，即使对于通量超过荧光激活细胞分选报告的那些的浓缩溶液也观察到96%或更高的值（5）。这种强大的非侵入性技术适用于集成（“芯片实验室”）微流体系统中的分选和分级，并可应用于胶体，分子和生物研究。
The response of a microscopic dielectric object to an applied light field can profoundly affect its kinetic motion(1). A classic example of this is an optical trap, which can hold a particle in a tightly focused light beam(2). Optical fields can also be used to arrange, guide or deflect particles in appropriate light-field geometries(3,4). Here we demonstrate an optical sorter for microscopic particles that exploits the interaction of particles-biological or otherwise-with an extended, interlinked, dynamically reconfigurable, three-dimensional optical lattice. The strength of this interaction with the lattice sites depends on the optical polarizability of the particles, giving tunable selection criteria. We demonstrate both sorting by size (of protein microcapsule drug delivery agents) and sorting by refractive index (of other colloidal particle streams). The sorting efficiency of this method approaches 100%, with values of 96% or more observed even for concentrated solutions with throughputs exceeding those reported for fluorescence-activated cell sorting(5). This powerful, non-invasive technique is suited to sorting and fractionation within integrated ('lab-on-a-chip') microfluidic systems, and can be applied in colloidal, molecular and biological research.