Biochemical detection of novel anaplastic lymphoma kinase proteins in tissue sections of anaplastic large cell lymphoma
Biochemical detection of novel anaplastic lymphoma kinase proteins in tissue sections of anaplastic large cell lymphoma
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DOI：
10.1016/s0002-9440(10)65421-1
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发表时间：
1999-06-01
影响因子：
6
通讯作者：
Mason, DY
中科院分区：
文献类型：
--
作者：
Pulford, K;Falini, B;Mason, DY
（2）; 5）易位，在许多T细胞和空细胞间变性大细胞淋巴瘤（ALCL）中发现，产生编码80-kd NPM-ALK蛋白的杂合基因。通常，肿瘤细胞显示出间变性淋巴瘤激酶（ALK）和核磷蛋白（NPM）N-末端的细胞核和细胞质标记。然而，10-20%的病例仅显示ALK的胞质标记，表明可能存在不涉及NPM基因的经典（2;5）易位变体。我们报告说，（使用蛋白质印迹和体外激酶测定）在7例此类ALCL病例中，检测分子量为85 kd、97 kd（1例显示（2;3）（p23;q21）易位），104 kd（1例携带（1;2）（q21;p23）易位）和113 kd的蛋白质中，有4个蛋白质检测到酪氨酸激酶活性，但NPM的N端部分未检测到。这些结果表明，表达ALK蛋白而非NPM-ALK的ALCL病例如何通过敏感的生化技术使用常规冷冻切片进行检测。
The (2;5) translocation, found in many T-cell and null cell anaplastic large cell lymphomas (ALCLs), creates a hybrid gene encoding the 80-kd NPM-ALK protein. Typically neoplastic cells show labeling of both nucleus and cytoplasm for anaplastic lymphoma kinase (ALK) and for the N-terminus of nucleophosmin (NPM). However, 10-20% of cases exhibit cytoplasmic labeling only for ALK, indicating the probable presence of variants of the classical (2;5) translocation that do not involve the NPM gene. We report the detection (using Western blotting and an in vitro kinase assay) in seven such ALCL cases, of ALK proteins with molecular masses of 85 kd, 97 kd (one case exhibiting a (2;3)(p23;q21) translocation), 104 kd (one case carried a (1;2)(q21;p23) translocation), and 113 kd, Tyrosine kinase activity was detected in four of these proteins, but the N-terminal portion of NPM could not be detected. These results show how ALCL cases that express ALK proteins other than NPM-ALK can be detected by sensitive biochemical techniques using routine cryostat sections.